ArticleFrontiers in microbiology2021
Influence of Filter Pore Size on Composition and Relative Abundance of Bacterial Communities and Select Host-Specific MST Markers in Coastal Waters of Southern Lake Michigan.
Article in Frontiers in microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed, 16 citations in OpenAlex.
- Dynamic Bacterial Communities, Resistome-Virulome Coupling, and Biomonitoring Paradigms at Direct Sea Discharge Outlets: An Integrated Microbiome Perspective for Coastal Pollution Control.Microorganisms · 2026Review
- Single-cell and bulk transcriptomic analyses uncover immune subtypes associated with programmed cell death features in intrahepatic cholangiocarcinoma.Scientific reports · 2026Article
- Protocol for efficient recovery of high-quality DNA from microbiome of marine invertebrates.Journal of microbiology (Seoul, Korea) · 2025Article
- Biogeographical Distribution of River Microbial Communities in Atlantic Catchments.Environmental microbiology reports · 2025Article
- Methodological differences between studies confound one-size-fits-all approaches to managing surface waterways for food and water safety.Applied and environmental microbiology · 2024Article
- Sediment-associated microbial community profiling: sample pre-processing through sequential membrane filtration for 16S rRNA amplicon sequencing.BMC microbiology · 2022Article
- Capturing marine microbiomes and environmental DNA: A field sampling guide.Frontiers in microbiology · 2022Review
- Combining tangential flow filtration and size fractionation of mesocosm water as a method for the investigation of waterborne coral diseases.Biology methods & protocols · 2022Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Water clarity is often the primary guiding factor in determining whether a prefiltration step is needed to increase volumes processed for a range of microbial endpoints. In this study, we evaluate the effect of filter pore size on the bacterial communities detected by 16S rRNA gene sequencing and incidence of two host-specific microbial source tracking (MST) markers in a range of coastal waters from southern Lake Michigan, using two independent data sets collected in 2015 (bacterial communities) and 2016-2017 (MST markers). Water samples were collected from river, shoreline, and offshore areas. For bacterial communities, each sample was filtered through a 5.0-μm filter, followed by filtration through a 0.22-μm filter, resulting in 70 and 143 filter pairs for bacterial communities and MST markers, respectively. Following DNA extraction, the bacterial communities were compared using 16S rRNA gene amplicons of the V3-V4 region sequenced on a MiSeq Illumina platform. Presence of human (
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