Evidence map›Paper›PMID 34335488›Full record

ArticleFrontiers in microbiology2021

Evaluation of Three Prokaryote Primers for Identification of Prokaryote Community Structure and Their Abode Preference in Three Distinct Wetland Ecosystems.

Kavita Kumari, Malay Naskar, Md Aftabuddin, Soma Das Sarkar, Bandana Das Ghosh, Uttam Kumar Sarkar, Subir Kumar Nag, Chayna Jana, Basanta Kumar Das

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Article in Frontiers in microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Kavita KumariAquatic Environmental Biotechnology and Nanotechnology Division, ICAR-Central Inland Fisheries Research Institute, Barrackpore, India.
Malay NaskarFisheries Resource Assessment and Informatics Division, ICAR-Central Inland Fisheries Research Institute, Barrackpore, India.
Md AftabuddinFisheries Resource Assessment and Informatics Division, ICAR-Central Inland Fisheries Research Institute, Barrackpore, India.
Soma Das SarkarFisheries Resource Assessment and Informatics Division, ICAR-Central Inland Fisheries Research Institute, Barrackpore, India.
Bandana Das GhoshICAR-Central Inland Fisheries Research Institute, Barrackpore, India.
Uttam Kumar SarkarReservoir and Wetland Fisheries Division, ICAR-Central Inland Fisheries Research Institute, Barrackpore, India.
Subir Kumar NagFisheries Resource Assessment and Informatics Division, ICAR-Central Inland Fisheries Research Institute, Barrackpore, India.
Chayna JanaFisheries Resource Assessment and Informatics Division, ICAR-Central Inland Fisheries Research Institute, Barrackpore, India.
Basanta Kumar DasICAR-Central Inland Fisheries Research Institute, Barrackpore, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The ultimate role of prokaryote (bacteria and archaea), the decomposer of the wetland ecosystem, depends on its community structure and its interaction with the environment. The present study has used three universal prokaryote primers to compare prokaryote community structure and diversity of three distinctly different wetlands. The study results revealed that α-diversity indices and phylogenetic differential abundance patterns did not differ significantly among primers, but they did differ significantly across wetlands. Microbial community composition revealed a distinct pattern for each primer in each wetland. Overall comparison of prokaryote communities in sediments of three wetlands revealed the highest prokaryote richness and diversity in Bhomra (freshwater wetland) followed by Malencho (brackish-water wetland) and East Kolkata wetland (EKW) (sewage-fed wetland). Indicator genus analysis identified 21, 4, and 29 unique indicator genera, having preferential abode for Bhomra, EKW, and Malencho, respectively. Prediction of potential roles of these microbes revealed a preference for sulfate-reducing microbes in Malencho and methanogens in Bhomra. The distinct phylogenetic differential abundance pattern, microbial abode preference, and their potential functional role predict ecosystem variables shaping microbial diversity. The variation in community composition of prokaryotes in response to ecosystem variables can serve as the most sensitive bioindicator of wetland ecosystem assessment and management.

Indexed as

brackishwater wetlandfloodplain wetlandhigh-throughput sequencingindicator taxasediment microbessewage-fed ecosystem

Identifiers

PMID34335488
PMCPMC8317468

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