ArticleHorticulture research2021
Illuminating the cells: transient transformation of citrus to study gene functions and organelle activities related to fruit quality.
Article in Horticulture research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 64 citations in OpenAlex.
- Identification and Functional Characterization of the GPAT Gene Family in Regulating Oil Biosynthesis in Olive.Plants (Basel, Switzerland) · 2026Article
- Dynamic chromatin regulatory programs of sucrose and citric acid metabolism during fruit ripening in Citrus.The Plant cell · 2026Article
- A Study on the Cell Layer Patterns of a Citrus Periclinal Chimera Reveals β-Cryptoxanthin Regulation in Citrus Fruits.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Transgene-free genome editing in citrus and poplar trees using positive and negative selection markers.Plant cell reports · 2025Article
- AP2 transcription factor CsAIL6 negatively regulates citric acid accumulation in citrus fruits by interacting with a WD40 protein CsAN11.Horticulture research · 2025Article
- The MADS-Box Transcription Factor CaRIN Positively Regulates Chlorophyll Degradation During Pepper (Plants (Basel, Switzerland) · 2025Article
- Improvement and application of vacuum-infiltration system in tomato.Horticulture research · 2024Article
- A high-efficiency transient expression system mediated by Agrobacterium tumefaciens in Spinacia oleracea leaves.Plant methods · 2024Article
- A rapid and efficient Agrobacterium-mediated transient transformation system in grape berries.Protoplasma · 2024Article
- Transient Expression Assay and Microscopic Observation in Kumquat Fruit.Bio-protocol · 2024Article
- Tracking organelle activities through efficient and stable root genetic transformation system in woody plants.Horticulture research · 2024Article
- Molecular characterization of oleosin genes in Cyperus esculentus, a Cyperaceae plant producing oil in underground tubers.Plant cell reports · 2023Article
- A Modified Method for Transient Transformation via Pollen Magnetofection inInternational journal of molecular sciences · 2023Article
- The establishment of transient expression systems and their application for gene function analysis of flavonoid biosynthesis in Carthamus tinctorius L.BMC plant biology · 2023Article
- Jasmonate activates a CsMPK6-CsMYC2 module that regulates the expression of β-citraurin biosynthetic genes and fruit coloration in orange (Citrus sinensis).The Plant cell · 2023Article
- CsMYB15 positively regulatesFrontiers in plant science · 2023Article
- Transcription factoraBIOTECH · 2022Article
- A New Method for Rapid Subcellular Localization and Gene Function Analysis in Cotton Based on Barley Stripe Mosaic Virus.Plants (Basel, Switzerland) · 2022Article
- Engineering the expression of plant secondary metabolites-genistein and scutellarin through an efficient transient production platform inFrontiers in plant science · 2022Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
Although multiple microscopic techniques have been applied to horticultural research, few studies of individual organelles in living fruit cells have been reported to date. In this paper, we established an efficient system for the transient transformation of citrus fruits using an Agrobacterium-mediated method. Kumquat (Fortunella crassifolia Swingle) was used; it exhibits higher transformation efficiency than all citrus fruits that have been tested and a prolonged-expression window. Fruits were transformed with fluorescent reporters, and confocal microscopy and live-cell imaging were used to study their localization and dynamics. Moreover, various pH sensors targeting different subcellular compartments were expressed, and the local pH environments in cells from different plant tissues were compared. The results indicated that vacuoles are most likely the main organelles that contribute to the low pH of citrus fruits. In summary, our method is effective for studying various membrane trafficking events, protein localization, and cell physiology in fruit and can provide new insight into fruit biology research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.