ArticleScientific reports2021
Photocrosslinkable liver extracellular matrix hydrogels for the generation of 3D liver microenvironment models.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- A comprehensive review of advances in hepatocyte microencapsulation: selecting materials and preserving cell viability.Frontiers in immunology · 2024Pooled it
- Approaches for recapitulating the extracellular matrix in organs-on-chip.Cell biomaterials · 2026Article
- From scaffold to function: A systematic review on dLECM-based hydrogels for liver tissue engineering - Fabrication, properties, and translational applications.Regenerative therapy · 2026Review
- Extracellular-Matrix-Based Materials from Decellularized Tissue: Opportunities, Challenges, and Future Directions in Regenerative Medicine.Advanced healthcare materials · 2026Review
- Bone-Derived dECM Hydrogels Support Tunable Microenvironments for In Vitro Osteogenic Differentiation.Advanced healthcare materials · 2026Article
- Development of Photocurable NorHA-dECM Hybrid Hydrogels to Study Cell-Matrix Interactions.ACS macro letters · 2025Article
- Engineered Hydrogels for Organoid Models of Human Nonalcoholic Fatty Liver Disease.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Tunable methacrylated decellularized heart matrix: a versatile scaffold for cardiac tissue engineering.Frontiers in bioengineering and biotechnology · 2025Article
- Value of a multi-indicator model combining Elast PQ technology, blood lipids, liver function, and uric acid for early diagnosis of alcoholic fatty liver disease.American journal of translational research · 2025Article
- Challenges ofFrontiers in cell and developmental biology · 2025Review
- The impact of matrix stiffness on hepatic cell function, liver fibrosis, and hepatocellular carcinoma-Based on quantitative data.Biophysics reviews · 2024Review
- Decellularized extracellular matrix biomaterials for regenerative therapies: Advances, challenges and clinical prospects.Bioactive materials · 2024Review
- Cancer cell response to extrinsic and intrinsic mechanical cue: opportunities for tumor apoptosis strategies.Regenerative biomaterials · 2024Review
- Chemical Modification of Human Decellularized Extracellular Matrix for Incorporation into Phototunable Hybrid-Hydrogel Models of Tissue Fibrosis.ACS applied materials & interfaces · 2023Article
- Review
- Hep3Gel: A Shape-Shifting Extracellular Matrix-Based, Three-Dimensional Liver Model Adaptable to Different Culture Systems.ACS biomaterials science & engineering · 2023Article
- Current Research Trends in the Application of In Vitro Three-Dimensional Models of Liver Cells.Pharmaceutics · 2022Review
- Engineering the Extracellular Matrix for Organoid Culture.International journal of stem cells · 2022Review
- A Novel Approach to Orthotopic Hepatocyte Transplantation Engineered With Liver Hydrogel for Fibrotic Livers, Enhancing Cell-Cell Interaction and Angiogenesis.Cell transplantationArticle
- Decellularization for the retention of tissue niches.Journal of tissue engineeringReview
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Liver extracellular matrix (ECM)-based hydrogels have gained considerable interest as biomimetic 3D cell culture environments to investigate the mechanisms of liver pathology, metabolism, and toxicity. The preparation of current liver ECM hydrogels, however, is based on time-consuming thermal gelation and limits the control of mechanical properties. In this study, we used detergent-based protocols to produce decellularized porcine liver ECM, which in turn were solubilized and functionalized with methacrylic anhydride to generate photocrosslinkable methacrylated liver ECM (LivMA) hydrogels. Firstly, we explored the efficacy of two protocols to decellularize porcine liver tissue using varying combinations of commonly used chemical agents such as Triton X-100, Sodium Dodecyl Sulphate (SDS) and Ammonium hydroxide. Then, we demonstrated successful formation of stable, reproducible LivMA hydrogels from both the protocols by photocrosslinking. The LivMA hydrogels obtained from the two decellularization protocols showed distinct mechanical properties. The compressive modulus of the hydrogels was directly dependent on the hydrogel concentration, thereby demonstrating the tuneability of mechanical properties of these hydrogels. Immortalized Human Hepatocytes cells were encapsulated in the LivMA hydrogels and cytocompatibility of the hydrogels was demonstrated after one week of culture. In summary, the LivMA hydrogel system provides a simple, photocrosslinkable platform, which can potentially be used to simulate healthy versus damaged liver for liver disease research, drug studies and cancer metastasis modelling.
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