ArticleProceedings of the National Academy of Sciences of the United States of America2021
Cell-free reconstitution reveals the molecular mechanisms for the initiation of secondary siRNA biogenesis in plants.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Molecular basis of plant DCL4 action that outcompetes DCL2.Nature plants · 2026Article
- The tasiR-ARF pathway in plants: origin, functions, and interplay of miR-390, tasiRNAs and ARF3.Plant biology (Stuttgart, Germany) · 2026Review
- Response to "The action of Arabidopsis DICER-LIKE2 in plant growth inhibition".The Plant cell · 2025Article
- Ribosome stalling-induced NIP5;1 mRNA decay triggers ARGONAUTE1-dependent transcription downregulation.Nucleic acids research · 2025Article
- The biogenesis, regulation and functions of transitive siRNA in plants.Acta biochimica et biophysica Sinica · 2024Review
- Asymmetric bulges within hairpin RNA transgenes influence small RNA size, secondary siRNA production and viral defence.Nucleic acids research · 2024Article
- Evidence for an RNAi-independent role of Arabidopsis DICER-LIKE2 in growth inhibition and basal antiviral resistance.The Plant cell · 2024Article
- The plant siRNA landscape.The Plant cell · 2024Review
- Article
- Hierarchical contribution of Argonaute proteins to antiviral protection.Journal of experimental botany · 2023Article
- Regulation of plant immunity via small RNA-mediated control of NLR expression.Journal of experimental botany · 2023Article
- The OsSGS3-tasiRNA-OsARF3 module orchestrates abiotic-biotic stress response trade-off in rice.Nature communications · 2023Article
- miRNA/siRNA-directed pathway to produce noncoding piRNAs from endogenous protein-coding regions ensuresScience advances · 2023Article
- Plant terminators: the unsung heroes of gene expression.Journal of experimental botany · 2023Review
- A beneficial fungal root endophyte triggers systemic RNA silencing and DNA methylation of a host reporter gene.RNA biology · 2023Article
- Article
- A spontaneous thermo-sensitive female sterility mutation in rice enables fully mechanized hybrid breeding.Cell research · 2022Article
- Functional specialization of monocot DCL3 and DCL5 proteins through the evolution of the PAZ domain.Nucleic acids research · 2022Article
- Nuclear and cytoplasmic RNA exosomes and PELOTA1 prevent miRNA-induced secondary siRNA production in Arabidopsis.Nucleic acids research · 2022Article
- Non-coding RNAs fine-tune the balance between plant growth and abiotic stress tolerance.Frontiers in plant science · 2022Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Secondary small interfering RNA (siRNA) production, triggered by primary small RNA targeting, is critical for proper development and antiviral defense in many organisms. RNA-dependent RNA polymerase (RDR) is a key factor in this pathway. However, how RDR specifically converts the targets of primary small RNAs into double-stranded RNA (dsRNA) intermediates remains unclear. Here, we develop an in vitro system that allows for dissection of the molecular mechanisms underlying the production of trans-acting siRNAs, a class of plant secondary siRNAs that play roles in organ development and stress responses. We find that a combination of the dsRNA-binding protein, SUPPRESSOR OF GENE SILENCING3; the putative nuclear RNA export factor, SILENCING DEFECTIVE5, primary small RNA, and Argonaute is required for physical recruitment of RDR6 to target RNAs. dsRNA synthesis by RDR6 is greatly enhanced by the removal of the poly(A) tail, which can be achieved by the cleavage at a second small RNA-binding site bearing appropriate mismatches. Importantly, when the complementarity of the base pairing at the second target site is too strong, the small RNA-Argonaute complex remains at the cleavage site, thereby blocking the initiation of dsRNA synthesis by RDR6. Our data highlight the light and dark sides of double small RNA targeting in the secondary siRNA biogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.