ArticleJournal of experimental botany2021
miR825-5p targets the TIR-NBS-LRR gene MIST1 and down-regulates basal immunity against Pseudomonas syringae in Arabidopsis.
Article in Journal of experimental botany, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Heat stress-induced remodelling of lipid and microRNA networks in Brassica napus germinated pollen and pollen-derived small extracellular vesicles.Journal of experimental botany · 2026Article
- Biotic Stress Resistance in Sweet Potato: Mechanisms, Perspectives, and Sustainable Production Strategies.Plants (Basel, Switzerland) · 2026Review
- ZmWRKY92-Mediated miR169s/NF-YA13 Module Confers Maize Resistance to Bipolaris maydis by Activating Flavonoid Biosynthesis.Molecular plant pathology · 2026Article
- miR825-5p-regulated TNLs govern Arabidopsis resistance to Tetranychus urticae and Pieris brassicae.The New phytologist · 2025Article
- Beware of Sealing Film of Petri Dishes!-Alters the Expression of a Large Number of Genes.International journal of molecular sciences · 2025Article
- Smi-miRmTERF regulates organelle development, retrograde signaling, secondary metabolism and immunity via targeting a subset of SmmTERFs in Salvia miltiorrhiza.Molecular horticulture · 2025Article
- Decoding the Dialog Between Plants and Arbuscular Mycorrhizal Fungi: A Molecular Genetic Perspective.Genes · 2025Review
- The biogenesis, regulation and functions of transitive siRNA in plants.Acta biochimica et biophysica Sinica · 2024Review
- Asymmetric bulges within hairpin RNA transgenes influence small RNA size, secondary siRNA production and viral defence.Nucleic acids research · 2024Article
- Transcriptional and epigenetic changes during tomato yellow leaf curl virus infection in tomato.BMC plant biology · 2023Article
- Regulation of plant immunity via small RNA-mediated control of NLR expression.Journal of experimental botany · 2023Article
- The genome of the Wollemi pine, a critically endangered "living fossil" unchanged since the Cretaceous, reveals extensive ancient transposon activity.bioRxiv : the preprint server for biology · 2023Article
- Genome-Wide Identification and Expression Analysis of the TIR-NBS-LRR Gene Family and Its Response to Fungal Disease in Rose (Biology · 2023Article
- Miniature Inverted-Repeat Transposable Elements: Small DNA Transposons That Have Contributed to PlantPlants (Basel, Switzerland) · 2023Review
- Recent Insights into Plant miRNA Biogenesis: Multiple Layers of miRNA Level Regulation.Plants (Basel, Switzerland) · 2023Review
- Functional Analysis of V2 Protein of Beet Curly Top Iran Virus.Plants (Basel, Switzerland) · 2022Article
- An Overview of PRR- and NLR-Mediated Immunities: Conserved Signaling Components across the Plant Kingdom That Communicate Both Pathways.International journal of molecular sciences · 2022Review
- Molecular innovations in plant TIR-based immunity signaling.The Plant cell · 2022Review
- New insight into the molecular mechanism of miR482/2118 during plant resistance to pathogens.Frontiers in plant science · 2022Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plants encode numerous intracellular receptors known as nucleotide-binding leucine-rich repeat receptors (NLRs) that recognize pathogen-derived effectors or their activity to activate defenses. miRNAs regulate NLR genes in many species, often triggering the production of phased siRNAs (phasiRNAs). Most such examples involve genes encoding NLRs carrying coiled-coil domains, although a few include genes encoding NLRs carrying a Toll/interleukin-1 domain (TNL). Here, we characterize the role of miR825-5p in Arabidopsis, using a combination of bioinformatics, transgenic plants with altered miRNA levels and/or reporters, small RNAs, and virulence assays. We demonstrate that miR825-5p down-regulates the TNL MIST1 by targeting for endonucleolytic cleavage the sequence coding for TIR2, a highly conserved amino acid motif, linked to a catalytic residue essential for immune function. miR825-5p acts as a negative regulator of basal resistance against Pseudomonas syringae. miR825-5p triggers the production from MIST1 of a large number of phasiRNAs that can mediate cleavage of both MIST1 and additional TNL gene transcripts, potentially acting as a regulatory hub. miR825-5p is expressed in unchallenged leaves and transcriptionally down-regulated in response to pathogen-associated molecular patterns (PAMPs). Our results show that miR825-5p, which is required for full expression of PAMP-triggered immunity, establishes a link between PAMP perception and expression of uncharacterized TNL genes.
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