ArticleBio-protocol2021
Analysis of Soluble Sugar Content in Minute Quantities of Rice Tissues by GC-MS.
Article in Bio-protocol, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Phytochemistry and insecticidal activity of six local plant extracts against Sitotroga Cerealella (Oliv.) (Lepidoptera: Gelechiidae) in stored wheat.BMC plant biology · 2025Article
- Two nucleotide sugar transporters are important for cell wall integrity and full virulence of Magnaporthe oryzae.Molecular plant pathology · 2023Article
- Grafting enhances drought tolerance by regulating and mobilizing proteome, transcriptome and molecular physiology in okra genotypes.Frontiers in plant science · 2023Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Soluble sugars play key roles in plant growth, development, and adaption to the environment. Characterizing sugar content profiling of plant tissues promotes our understanding of the mechanisms underlying these plant processes. Several technologies have been developed to quantitate soluble sugar content in plant tissues; however, it is difficult with only minute quantities of plant tissues available. Here, we provide a detailed protocol for gas chromatography mass spectrometry (GC-MS)-based soluble sugar profiling of rice tissues that offers a good balance of sensitivity and reliability, and is considerably more sensitive and accurate than other reported methods. We summarize all the steps from sample collection and soluble sugar extraction to derivatization procedures of the soluble extracted sugars, instrumentation settings, and data analysis.
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