ArticleScientific reports2021
CRISPR/Cas9-based genetic screen of SCNT-reprogramming resistant genes identifies critical genes for male germ cell development in mice.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 20 citations in OpenAlex.
- Genome-Wide Association Study Reveals Genetic Loci Associated with Body Measurement Traits in Yanqi Horses.Animals : an open access journal from MDPI · 2026Article
- Integrative bioinformatics analyses of mitochondrial dysfunction-related genes in human non-obstructive azoospermia.Scientific reports · 2026Article
- Quantitative dissection of sexual dimorphism in mice through Y-linked gene knockouts and multivariate phenotyping.Scientific reports · 2026Article
- NSUN7 is a catalytically inactive RNA mNature communications · 2025Article
- Whole genome sequencing approach for the detection of rare protein-altering variants associated with familial male infertility.The Egyptian journal of medical human genetics · 2025Article
- Meiotic cohesin RAD21L shapes 3D genome structure and transcription in the male germline.Science advances · 2025Article
- CRISPR-Cas9 in the Tailoring of Genetically Engineered Animals.Current issues in molecular biology · 2025Review
- Promyelocytic Leukemia Protein (PML) Regulates Stem Cell Pluripotency Through Novel Sumoylation Targets.International journal of molecular sciences · 2025Article
- Reduction of H3K9 methylation by G9a inhibitors improves the development of mouse SCNT embryos.Stem cell reports · 2024Article
- Disruption of testis-enriched cytochrome c oxidase subunit COX6B2 but not COX8C leads to subfertility.Experimental animals · 2024Article
- scapGNN: A graph neural network-based framework for active pathway and gene module inference from single-cell multi-omics data.PLoS biology · 2023Article
- Insights on the Role of α- and β-Tubulin Isotypes in Early Brain Development.Molecular neurobiology · 2023Review
- VASA protein and gene expression analysis of human non-obstructive azoospermia and normal by immunohistochemistry, immunocytochemistry, and bioinformatics analysis.Scientific reports · 2022Article
- Suppression of endogenous retroviral enhancers in mouse embryos derived from somatic cell nuclear transfer.Frontiers in genetics · 2022Article
- Evolution of a New Testis-Specific Functional Promoter Within the Highly ConservedFrontiers in genetics · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Male germ cells undergo complex developmental processes eventually producing spermatozoa through spermatogenesis, although the molecular mechanisms remain largely elusive. We have previously identified somatic cell nuclear transfer-reprogramming resistant genes (SRRGs) that are highly enriched for genes essential for spermatogenesis, although many of them remain uncharacterized in knockout (KO) mice. Here, we performed a CRISPR-based genetic screen using C57BL/6N mice for five uncharacterized SRRGs (Cox8c, Cox7b2, Tuba3a/3b, Faiml, and Gm773), together with meiosis essential gene Majin as a control. RT-qPCR analysis of mouse adult tissues revealed that the five selected SRRGs were exclusively expressed in testis. Analysis of single-cell RNA-seq datasets of adult testis revealed stage-specific expression (pre-, mid-, or post-meiotic expression) in testicular germ cells. Examination of testis morphology, histology, and sperm functions in CRISPR-injected KO adult males revealed that Cox7b2, Gm773, and Tuba3a/3b are required for the production of normal spermatozoa. Specifically, Cox7b2 KO mice produced poorly motile infertile spermatozoa, Gm773 KO mice produced motile spermatozoa with limited zona penetration abilities, and Tuba3a/3b KO mice completely lost germ cells at the early postnatal stages. Our genetic screen focusing on SRRGs efficiently identified critical genes for male germ cell development in mice, which also provides insights into human reproductive medicine.
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