Evidence map›Paper›PMID 34325785›Full record

ArticleMethods in enzymology2021

Synthesis and study of macrocyclic β-hairpin peptides for investigating amyloid oligomers.

Gretchen Guaglianone, Adam G Kreutzer, James S Nowick

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in enzymology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Gretchen GuaglianoneDepartment of Chemistry, University of California, Irvine, CA, United States.
Adam G KreutzerDepartment of Chemistry, University of California, Irvine, CA, United States.
James S NowickDepartment of Chemistry, University of California, Irvine, CA, United States; Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, CA, United States. Electronic address: jsnowick@uci.edu.
University of California, Irvine · US

Funding

Antibodies Against Conformationally Defined Amyloid Oligomers in Alzheimer’s DiseaseRF1AG062296 · NIA · UNIVERSITY OF CALIFORNIA-IRVINE · PI NOWICK, JAMES S · 2018 to 2018
$3.0M
Purchase of an Microwave Peptide SynthesizerR01GM097562 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI NOWICK, JAMES S · 2012 to 2019
$2.5M
NIA NIH HHS RF1 AG062296NIGMS NIH HHS R01 GM097562
6 · The paper itself

Abstract

Chemically constrained peptides that self-assemble can be used to better understand the molecular basis of amyloid diseases. The formation of small assemblies of the amyloidogenic peptides and proteins, termed oligomers, is central to amyloid diseases. The use of chemical model systems can help provide insights into the structures and interactions of amyloid oligomers, which are otherwise difficult to study. This chapter describes the use of macrocyclic β-hairpin peptides as model systems to study amyloid oligomers. The first part of the chapter describes the chemical synthesis of the macrocyclic β-hairpin peptides and covalent assemblies thereof. The second part of the chapter describes the characterization of the oligomers formed by the macrocyclic β-hairpin peptides, focusing on SDS-PAGE, size-exclusion chromatography (SEC), and X-ray crystallography. The procedures provided focus on the β-amyloid peptide, but these strategies are applicable to a broad range of amyloid-derived peptides and proteins.

Indexed as

AmyloidAmyloid beta-PeptidesCrystallography, X-RayModels, MolecularAmyloidAmyloid beta-PeptidesAmyloid diseasesAmyloid oligomersMacrocyclic β-hairpin peptidesPeptide X-ray crystallographySDS-PAGESize-exclusion chromatography

Identifiers

PMID34325785
PMCPMC9053554
OpenAlexW3183689812

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.