ArticlePlant methods2021
Efficient isolation and purification of tissue-specific protoplasts from tea plants (Camellia sinensis (L.) O. Kuntze).
Article in Plant methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 37 citations in OpenAlex.
- An efficient method for tissue-specific protoplast isolation suitable for single-cell RNA sequencing and transient gene expression analysis in saffron (Crocus sativus L.).BMC biotechnology · 2026Article
- Protoplast isolation and transient gene expression in Suaeda aralocaspica, a halophyte with single-cell CPlant methods · 2026Article
- PVP-40 mediated enhancement of mesophyll protoplast yield and viability for transient gene expression in black huckleberry.Plant methods · 2025Article
- Targeted genome modification in protoplasts of a tea cultivar Kolkhida using RNA-guided Cas9 endonuclease.AoB PLANTS · 2025Article
- Establishment of Transient Transformation Systems in Welsh Onion (Plants (Basel, Switzerland) · 2025Article
- Optimization of Protoplast Preparation and Establishment of PEG-Mediated Genetic Transformation Method inJournal of fungi (Basel, Switzerland) · 2025Article
- Development of Protoplast-Based Gene Editing System for Areca Palm.Plants (Basel, Switzerland) · 2025Article
- Optimization of preparation conditions for Salsola laricifolia protoplasts using response surface methodology and artificial neural network modeling.Plant methods · 2024Article
- Using A Protoplast Transformation System to Enable Functional Studies inInternational journal of molecular sciences · 2023Article
- Opportunities and challenges in the application of single-cell and spatial transcriptomics in plants.Frontiers in plant science · 2023Review
- Cotyledon peeling method for passion fruit protoplasts: a versatile cell system for transient gene expression in passion fruitFrontiers in plant science · 2023Article
- Isolation, purification and PEG-mediated transient expression of mesophyll protoplasts in Camellia oleifera.Plant methods · 2022Article
- Single-cell transcriptome atlas reveals developmental trajectories and a novel metabolic pathway of catechin esters in tea leaves.Plant biotechnology journal · 2022Article
- An integrated physiology, cytology, and proteomics analysis reveals a network of sugarcane protoplast responses to enzymolysis.Frontiers in plant science · 2022Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundPlant protoplasts constitute unique single-cell systems that can be subjected to genomic, proteomic, and metabolomic analysis. An effective and sustainable method for preparing protoplasts from tea plants has yet to be established. The protoplasts were osmotically isolated, and the isolation and purification procedures were optimized. Various potential factors affecting protoplast preparation, including enzymatic composition and type, enzymatic hydrolysis duration, mannitol concentration in the enzyme solution, and iodixanol concentration, were evaluated.
resultsThe optimal conditions were 1.5% (w/v) cellulase and 0.4-0.6% (w/v) macerozyme in a solution containing 0.4 M mannitol, enzymatic hydrolysis over 10 h, and an iodixanol concentration of 65%. The highest protoplast yield was 3.27 × 10
conclusionsIn summary, we reported on a simple, efficient method for preparing protoplasts of whole-organ tissue from tea plant. The findings are expected to contribute to the rapid development of tea plant biology.
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