ArticleMethods in molecular biology (Clifton, N.J.)2021
Direct Conversion of Human Fibroblasts to Induced Neurons.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Cellular Senescence of Patient-derived Fibroblasts Reveals the Mid-old Stage as a Critical Window for Transcriptomic Signatures Linked to Alzheimer's Disease Biomarkers and Classification.Clinical psychopharmacology and neuroscience : the official scientific journal of the Korean College of Neuropsychopharmacology · 2026Article
- Using iPSC models to examine neuron-glia interactions in neurodegenerative diseases.Bioscience reports · 2026Review
- Transcriptional signature of induced neurons differentiates virologically suppressed people with HIV from people without HIV.JCI insight · 2026Article
- The complement cascade in Alzheimer's disease: modern implications of an ancient immune protagonist.Molecular neurodegeneration · 2025Review
- The mTOR signaling pathway mediates the phenotypic switch between adipocytes and osteocytes in mice with obesity-related osteoporosis.European journal of medical research · 2025Article
- Tracing mitochondrial marks of neuronal aging in iPSCs-derived neurons and directly converted neurons.Communications biology · 2025Article
- A Transcriptional Signature of Induced Neurons Differentiates Virologically Suppressed People Living With HIV from People Without HIV.bioRxiv : the preprint server for biology · 2024Article
- RNA variant assessment using transactivation and transdifferentiation.American journal of human genetics · 2024Article
- From Young to Old: Mimicking Neuronal Aging in Directly Converted Neurons from Young Donors.Cells · 2024Article
- Microglial lipid droplet accumulation in tauopathy brain is regulated by neuronal AMPK.Cell metabolism · 2024Article
- Recent advances and current challenges of new approach methodologies in developmental and adult neurotoxicity testing.Archives of toxicology · 2024Review
- Druggable transcriptomic pathways revealed in Parkinson's patient-derived midbrain neurons.NPJ Parkinson's disease · 2022Article
- Chemical Replacement of Noggin with Dorsomorphin Homolog 1 for Cost-Effective Direct Neuronal Conversion.Cellular reprogramming · 2022Article
Corrections and comments
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Authors and funding
6 authors.
Funding
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Abstract
Progressive aging is a physiological process that represents a central risk factor for the development of several human age-associated chronic diseases, including neurodegenerative diseases. A major focus in biomedical research is the pursuit for appropriate model systems to better model the biology of human aging and the interface between aging and disease mechanisms. Direct conversion of human fibroblasts into induced neurons (iNs) has emerged as a novel technology for the in vitro modeling of age-dependent neurological diseases. Similar to other cellular reprogramming techniques, e.g., iPSC-based cellular reprograming, direct conversion relies on the ectopic overexpression of transcription factors, typically including well-known pioneer factors. However, in contrast to alternative technologies to generate neurons, the entire process of direct conversion bypasses any proliferative or stem cell-like stage, which in fact renders it the unique aptitude of preserving age-associated hallmarks from the initial fibroblast source. In this chapter, we introduce direct conversion as a practical and easy-to-approach disease model for aging and neurodegenerative disease research. A focus here is to provide a stepwise protocol for the efficient and highly reproducible generation of iNs from adult dermal fibroblasts from human donors.
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Registered trials
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