ArticleThe Science of the total environment2021
An alternative approach for bioanalytical assay optimization for wastewater-based epidemiology of SARS-CoV-2.
Article in The Science of the total environment, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 3 of them syntheses that pooled it.
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Who cites it
20 citing papers in PubMed, 3 syntheses or guidelines pooled it, 36 citations in OpenAlex.
- A Systematic Review of Methodological Approaches to SARS-CoV-2 Wastewater Surveillance.Viruses · 2026Pooled it
- Recent progress on wastewater-based epidemiology for COVID-19 surveillance: A systematic review of analytical procedures and epidemiological modeling.The Science of the total environment · 2023Pooled it
- Factors influencing SARS-CoV-2 RNA concentrations in wastewater up to the sampling stage: A systematic review.The Science of the total environment · 2022Pooled it
- Variabilities in N2 and E Gene Concentrations in a SARS-CoV-2 Wastewater Multiplex Assay.Microorganisms · 2025Article
- Impact of Sample Storage Time and Temperature on the Stability of Respiratory Viruses and Enteric Viruses in Wastewater.Microorganisms · 2024Article
- The first detection of SARS-CoV-2 RNA in the wastewater of Bucharest, Romania.Scientific reports · 2024Article
- Comparison of Different Reverse Transcriptase-Polymerase Chain Reaction-Based Methods for Wastewater Surveillance of SARS-CoV-2: Exploratory Study.JMIR public health and surveillance · 2024Article
- A scoping review of global SARS-CoV-2 wastewater-based epidemiology in light of COVID-19 pandemic.Heliyon · 2024Article
- Wastewater-based surveillance as a tool for public health action: SARS-CoV-2 and beyond.Clinical microbiology reviews · 2024Review
- Regional and temporal differences in the relation between SARS-CoV-2 biomarkers in wastewater and estimated infection prevalence - Insights from long-term surveillance.The Science of the total environment · 2023Article
- Persistence of endogenous RNA biomarkers of SARS-CoV-2 and PMMoV in raw wastewater: Impact of temperature and implications for wastewater-based epidemiology.The Science of the total environment · 2023Article
- Biomarker selection strategies based on compound stability in wastewater-based epidemiology.Environmental science and pollution research international · 2023Review
- Evaluation of viral concentration and extraction methods for SARS-CoV-2 recovery from wastewater using droplet digital and quantitative RT-PCR.Case studies in chemical and environmental engineering · 2022Article
- Comparison of RT-dPCR and RT-qPCR and the effects of freeze-thaw cycle and glycine release buffer for wastewater SARS-CoV-2 analysis.Scientific reports · 2022Article
- Comparison of RT-qPCR and RT-dPCR Platforms for the Trace Detection of SARS-CoV-2 RNA in Wastewater.ACS ES&T water · 2022Article
- SARS-CoV-2 Surveillance in Belgian Wastewaters.Viruses · 2022Article
- SARS-CoV-2 surveillance in Norway rats (Rattus norvegicus) from Antwerp sewer system, Belgium.Transboundary and emerging diseases · 2022Article
- Article
- Prevalence of SARS-CoV-2 genes in water reclamation facilities: From influent to anaerobic digester.The Science of the total environment · 2021Article
- Mathematical modeling based on RT-qPCR analysis of SARS-CoV-2 in wastewater as a tool for epidemiology.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wastewater-based epidemiology of SARS-CoV-2 could play a role in monitoring the spread of the virus in the population and controlling possible outbreaks. However, sensitive sample preparation and detection methods are necessary to detect trace levels of SARS-CoV-2 RNA in influent wastewater (IWW). Unlike predecessors, method optimization of a SARS-CoV-2 RNA concentration and detection procedure was performed with IWW samples with high viral SARS-CoV-2 RNA loads. This is of importance since the SARS-CoV-2 genome in IWW might have already been subject to in-sewer degradation into smaller genome fragments or might be present in a different form (e.g. cell debris, …). Centricon Plus-70 (100 kDa) centrifugal filter devices resulted in the lowest and most reproducible Ct-values for SARS-CoV-2 RNA. Lowering the molecular weight cut-off did not improve our limit of detection and quantification (approximately 10
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