ArticleOpen forum infectious diseases2021
Use of Metagenomic Next-Generation Sequencing to Identify Pathogens in Pediatric Osteoarticular Infections.
Article in Open forum infectious diseases, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
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Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- The epidemiology and management of chronic osteomyelitis in pediatrics - A systematic review.PloS one · 2025Pooled it
- How Should Bacteriological Sampling Be Stratified in Paediatric Septic Arthritis? A Narrative Review with a Proposed Risk-Stratified Framework.Antibiotics (Basel, Switzerland) · 2026Review
- Clinical Characteristics and Outcomes of Culture-positive Versus Culture-negative Bone and Joint Infections in Pediatric Patients: A 10-Year Multicenter Study From Hungary.The Pediatric infectious disease journal · 2026Observational
- It is time to have a molecular test for diagnosingJournal of clinical microbiology · 2026Article
- A model for predicting the likelihood of negative culture results in children with osteoarticular infections.European journal of medical research · 2026Article
- Spinal infections.Frontiers in neuroscience · 2026Review
- Predictive value of AFR and UCR for the methicillin-resistant Staphylococcus aureus infection in children with bone and joint infection.Scientific reports · 2025Article
- Fastidious or rarely isolated gram-negative rods with a particular focus onClinical microbiology reviews · 2025Review
- Identifying septic arthritis superimposed on crystalline arthropathy: Is synovial cell count the answer?Journal of clinical orthopaedics and trauma · 2025Article
- Diagnosis ofFrontiers in pediatrics · 2025Article
- Identification of Pathogens in HIV-Infected Patients Using Metagenomic Next-Generation Sequencing (mNGS) as Compared to Conventional Microbiological Tests (CMTs).Infection and drug resistance · 2025Article
- Clinical characteristics and prognosis of children with culture-negative osteoarticular infections: a meta-analysis based on cohort studies.EFORT open reviews · 2025Article
- Article
- Duration of onset, body temperature and C-reactive protein can be used to predict the results of pus culture in children with acute osteomyelitis of long bones.Italian journal of pediatrics · 2024Article
- Effect of Preoperative Antibiotic Therapy on Operative Culture Yield for Diagnosis of Native Joint Septic Arthritis.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2024Article
- Bacteriological diagnosis of osteoarticular infections caused byFrontiers in pediatrics · 2024Review
- Laboratory diagnostics for primary spinal infections in pediatric and adult populations: a narrative review.North American Spine Society journal · 2023Article
- Metagenomic next-generation sequencing in osteoarticular infections with a focus on pediatrics: current concepts and clinical applications.EFORT open reviews · 2023Review
- Clinical Evaluation of Metagenomic Next-Generation Sequencing for the detection of pathogens in BALF in severe community acquired pneumonia.Italian journal of pediatrics · 2023Article
- Deciphering Microbiota of Acute Upper Respiratory Infections: A Comparative Analysis of PCR and mNGS Methods for Lower Respiratory Trafficking Potential.Advances in respiratory medicine · 2023Article
Corrections and comments
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Authors and funding
16 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOsteoarticular infections (OAIs) are frequently encountered in children. Treatment may be guided by isolation of a pathogen; however, operative cultures are often negative. Metagenomic next-generation sequencing (mNGS) allows for broad and sensitive pathogen detection that is culture-independent. We sought to evaluate the diagnostic utility of mNGS in comparison to culture and usual care testing to detect pathogens in acute osteomyelitis and/or septic arthritis in children.
methodsThis was a single-site study to evaluate the use of mNGS in comparison to culture to detect pathogens in acute pediatric osteomyelitis and/or septic arthritis. Subjects admitted to a tertiary children's hospital with suspected OAI were eligible for enrollment. We excluded subjects with bone or joint surgery within 30 days of admission or with chronic osteomyelitis. Operative samples were obtained at the surgeon's discretion per standard care (fluid or tissue) and based on imaging and operative findings. We compared mNGS to culture and usual care testing (culture and polymerase chain reaction [PCR]) from the same site.
resultsWe recruited 42 subjects over the enrollment period. mNGS of the operative samples identified a pathogen in 26 subjects compared to 19 subjects in whom culture identified a pathogen. In 4 subjects, mNGS identified a pathogen where combined usual care testing (culture and PCR) was negative. Positive predictive agreement and negative predictive agreement both were 93.0% for mNGS.
conclusionsIn this single-site prospective study of pediatric OAI, we demonstrated the diagnostic utility of mNGS testing in comparison to culture and usual care (culture and PCR) from operative specimens.
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