Evidence map›Paper›PMID 34321981›Full record

ArticleJournal of separation science2021

Microchip Electrophoresis Assay for Calmodulin Binding Proteins.

Thushara N Samarasinghe, Yong Zeng, Carey K Johnson

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of separation science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 52% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Thushara N SamarasingheDepartment of Chemistry, University of Kansas, Lawrence, Kansas, USA.
Yong ZengDepartment of Chemistry, University of Kansas, Lawrence, Kansas, USA.
Carey K JohnsonDepartment of Chemistry, University of Kansas, Lawrence, Kansas, USA.
University of Kansas · US

Funding

Understanding the mechanobiology of stem cells in a microengineered 3D cardiac tissue environment with cardiomyopathyP20GM103638 · NIGMS · UNIVERSITY OF KANSAS LAWRENCE · PI LUNTE, SUSAN M · 2012 to 2021
$22.1M
NIGMS NIH HHS P20 GM103638
6 · The paper itself

Abstract

The calcium signaling protein calmodulin regulates numerous intracellular processes. We introduce a sensitive microchip assay to separate and detect calmodulin binding proteins. The assay utilizes an optimized microchip electrophoresis protein separation platform with laser-induced fluorescence detection. Fluorescence-labeled calmodulin modified with a photoreactive diazirine crosslinker allowed selective detection of calmodulin binding proteins. We demonstrate successful in

Indexed as

Electrophoresis, MicrochipCalmodulin-Binding ProteinsCalmodulin-Binding Proteinscalmodulin binding proteincolloidal arraymicellar electrokinetic chromatographymicrochip electrophoresisphoto-crosslinking

Identifiers

PMID34321981
PMCPMC8312402
OpenAlexW3111425857

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.