ArticleAIChE journal. American Institute of Chemical Engineers2021
Natural deep eutectic systems for nature-inspired cryopreservation of cells.
Article in AIChE journal. American Institute of Chemical Engineers, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 12 citations in OpenAlex.
- Natural killer cell cryopreservation: Influence of composition, temperature, and granules on post-thaw survival and function.Molecular therapy. Advances · 2026Article
- Evaluation of Deep Eutectic Solvents for Cryopreservation of the Fish PathogenMethods and protocols · 2026Article
- Thermodynamic Principles of Emerging Cryopreservation Technologies.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Incorporation of a Natural Deep Eutectic Solvent-Based System as a Cryoprotectant in Solid Lipid Nanoparticles: Advancing toward Industrial Scalability.ACS nanoscience Au · 2025Article
- Computational Design of Natural Deep Eutectic Systems Using COSMO-RS for Ice Control Applications.ACS sustainable chemistry & engineering · 2025Article
- Effects of Different Trehalose and Sorbitol Impregnation Methods on Freeze-Thaw Damage to Potato Slices.Foods (Basel, Switzerland) · 2025Article
- DMSO-free cryopreservation of hiPSC-derived cardiomyocytes: low temperature characterization and protocol development.Stem cell research & therapy · 2025Article
- Deep Eutectic Systems: A Game Changer for Marine Bioactives Recovery.Marine drugs · 2025Review
- A size-dependent ideal solution model for liquid-solid phase equilibria prediction in aqueous organic solutions.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Exploring Deep Eutectic Solvents as Pharmaceutical Excipients: Enhancing the Solubility of Ibuprofen and Mefenamic Acid.Pharmaceuticals (Basel, Switzerland) · 2024Article
- Pharmaceutical applications of therapeutic deep eutectic systems (THEDES) in maximising drug delivery.Heliyon · 2024Review
- From Nature to Innovation: The Uncharted Potential of Natural Deep Eutectic Solvents.Molecules (Basel, Switzerland) · 2023Review
- Characterization of eutectic mixtures of sugars and sugar-alcohols for cryopreservation.Journal of molecular liquids · 2023Article
- Stabilization of insect cell membranes and soluble enzymes by accumulated cryoprotectants during freezing stress.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Natural Cryoprotective and Cytoprotective Agents in Cryopreservation: A Focus on Melatonin.Molecules (Basel, Switzerland) · 2022Review
- Differentiation of Human iPS Cells Into Sensory Neurons Exhibits Developmental Stage-Specific Cryopreservation Challenges.Frontiers in cell and developmental biology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Natural deep eutectic systems (NADES) are emerging as potential cryoprotective agents (CPA) for cell preservation. In this investigation, we develop an optimized CPA formulation using trehalose-glycerol NADES (T:G) diluted in Normosol-R and supplemented with isoleucine. Differential scanning calorimetry (DSC) is used to define the thermophysical properties of NADES-based solutions, and Raman spectroscopy is used to characterize the effect of NADES on ice formation and hydrogen bonding. Jurkat cells are cryopreserved in each solution, and post-thaw cell recovery, apoptosis, and growth are quantified. Raman spectra and heat maps show that NADES suppresses both ice formation and dehydration of the nonfrozen region. Supplementing NADES with isoleucine does not affect the solution's thermophysical properties but significantly improves the cells' survival and proliferation post-thaw. The study indicates that thermophysical properties of CPA solutions alone cannot predict optimal cell survival, suggesting that stabilization of biological structures by CPAs may play a role in successful cryopreservation.
Indexed as
Identifiers
34321676PMC8315112W3089835622What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.