ArticleNature communications2021
Multifocal imaging for precise, label-free tracking of fast biological processes in 3D.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Precise 3D Tracking of Highly Non-Planar Eukaryotic Flagellar Beating Patterns Using Digital Holographic Microscopy.Small methods · 2026Article
- Super Time-Resolved Tomography.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Swimming by Spinning: Spinning-Top Type Rotations Regularize Sperm Swimming Into Persistently Progressive Paths in 3D.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- AI-based hardware and software tools in microscopy to boost research in immunology and virology.Frontiers in immunology · 2025Review
- Development of crystal optics for X-ray multi-projection imaging for synchrotron and XFEL sources.Journal of synchrotron radiation · 2024Article
- Multifocal microscopy for functional imaging of neural systems.Neurophotonics · 2024Article
- Fluid flow reconstruction around a free-swimming sperm in 3D.bioRxiv : the preprint server for biology · 2024Article
- Article
- Article
- Multifocal imaging for precise, label-free tracking of fast biological processes in 3D.Nature communications · 2021Article
- Rapid multi-plane phase-contrast microscopy reveals torsional dynamics in flagellar motion.Biomedical optics express · 2021Article
- CiliaQ: a simple, open-source software for automated quantification of ciliary morphology and fluorescence in 2D, 3D, and 4D images.The European physical journal. E, Soft matter · 2021Article
- Modelling Motility: The Mathematics of Spermatozoa.Frontiers in cell and developmental biology · 2021Review
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many biological processes happen on a nano- to millimeter scale and within milliseconds. Established methods such as confocal microscopy are suitable for precise 3D recordings but lack the temporal or spatial resolution to resolve fast 3D processes and require labeled samples. Multifocal imaging (MFI) allows high-speed 3D imaging but is limited by the compromise between high spatial resolution and large field-of-view (FOV), and the requirement for bright fluorescent labels. Here, we provide an open-source 3D reconstruction algorithm for multi-focal images that allows using MFI for fast, precise, label-free tracking spherical and filamentous structures in a large FOV and across a high depth. We characterize fluid flow and flagellar beating of human and sea urchin sperm with a z-precision of 0.15 µm, in a volume of 240 × 260 × 21 µm, and at high speed (500 Hz). The sampling volume allowed to follow sperm trajectories while simultaneously recording their flagellar beat. Our MFI concept is cost-effective, can be easily implemented, and does not rely on object labeling, which renders it broadly applicable.
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