Evidence map›Paper›PMID 34321357›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2021

Architecture of cell-cell junctions in situ reveals a mechanism for bacterial biofilm inhibition.

Charlotte E Melia, Jani R Bolla, Stefan Katharios-Lanwermeyer, Daniel B Mihaylov, Patrick C Hoffmann, Jiandong Huo, Michael R Wozny, Louis M Elfari, Jan Böhning, Ashleigh N Morgan and 5 more

Open access · hybridAbstract read
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed, 1 pooled it
2.9field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.

  1. Molecular imaging of bacterial biofilms-a systematic review.Critical reviews in microbiology · 2024
    Pooled it
  2. Structural basis of biofilm formation mediated by thebioRxiv : the preprint server for biology · 2026
    Article
  3. Article
  4. Cryogenic electron tomography by the numbers: Charting underexplored lineages in structural cell biology.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  5. Case Study: Illuminating the Nanoscale World of Microbiology.Methods in molecular biology (Clifton, N.J.) · 2026
    Review
  6. Molecular tags for electron cryo-tomography.Emerging topics in life sciences · 2025
    Review
  7. Article
  8. Article
  9. Review
  10. Article
  11. Structural basis for immune cell binding ofProceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  12. Article
  13. Article
  14. Article
  15. Review
  16. Article
  17. Charting the molecular landscape of the cell.Structure (London, England : 1993) · 2023
    Review
  18. Review
  19. Article
  20. How to study biofilms: technological advancements in clinical biofilm research.Frontiers in cellular and infection microbiology · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 4 institutions in 2 countries.

Charlotte E MeliaSir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom.
Jani R BollaPhysical and Theoretical Chemistry Laboratory, University of Oxford, Oxford OX1 3TA, United Kingdom.ORCID 0000-0003-4346-182X
Stefan Katharios-LanwermeyerDepartment of Microbiology and Immunology, Geisel School of Medicine, Dartmouth College, Hanover, NH 03755.
Daniel B MihaylovSir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom.
Patrick C HoffmannCell Biology Division, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom.
Jiandong HuoProtein Production Facility United Kingdom, Rosalind Franklin Institute - Research Complex at Harwell, Didcot OX11 0FA, United Kingdom.
Michael R WoznyCell Biology Division, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom.ORCID 0000-0001-9359-3287
Louis M ElfariSir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom.
Jan BöhningSir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom.ORCID 0000-0002-6242-1041
Ashleigh N MorganSir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom.
Charlie J HitchmanSir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom.
Raymond J OwensProtein Production Facility United Kingdom, Rosalind Franklin Institute - Research Complex at Harwell, Didcot OX11 0FA, United Kingdom.
Carol V RobinsonPhysical and Theoretical Chemistry Laboratory, University of Oxford, Oxford OX1 3TA, United Kingdom.ORCID 0000-0001-7829-5505
George A O'TooleDepartment of Microbiology and Immunology, Geisel School of Medicine, Dartmouth College, Hanover, NH 03755.
Tanmay A M BharatSir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom; tanmay.bharat@path.ox.ac.uk.ORCID 0000-0002-0168-0277
University of Oxford · GBCentre for Human Genetics · GBDartmouth College · USMRC Laboratory of Molecular Biology · GB

Funding

cdiGMP regulation of Biofilm FormationR37AI083256 · NIAID · DARTMOUTH COLLEGE · PI O'TOOLE, GEORGE A. · 2014 to 2023
$4.7M
Molecular mechanism regulating periplasmic proteolysis in bacterial pathogenesisR01GM123609 · NIGMS · CORNELL UNIVERSITY · PI O'TOOLE, GEORGE A., SONDERMANN, HOLGER · 2017 to 2020
$1.3M
Medical Research Council MC_UP_1201/10Medical Research Council MR/N020413/1NIAID NIH HHS R37 AI083256NIGMS NIH HHS R01 GM123609Wellcome Trust 202231/Z/16/Z
6 · The paper itself

Abstract

Many bacteria, including the major human pathogen

Indexed as

Adhesins, BacterialBacterial AdhesionBiofilmsCell MembraneExtracellular MatrixGene Expression Regulation, BacterialPseudomonas aeruginosaSingle-Domain AntibodiesAdhesins, BacterialCdrA protein, Pseudomonas aeruginosaSingle-Domain Antibodiesantibioticsbiofilmscryo-EMin situ imagingnanobody

Identifiers

PMID34321357
PMCPMC8346871
OpenAlexW3186110890

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.