ArticleParasites & vectors2021
A multiplex PCR assay for six Aedini species, including Aedes albopictus.
Article in Parasites & vectors, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Molecular exploration of mosquitoes and their vector-borne pathogens in urban water environments in Benin.Scientific reports · 2026Article
- Global environmental niche dynamics of two invasive Aedes mosquitoes, Aedes japonicus and Aedes koreicus (Diptera: Culicidae), using comprehensive native and non-native occurrence data.Parasites & vectors · 2026Article
- Comparison of a multiplex PCR with DNA barcoding for identification of container breeding mosquito species.Parasites & vectors · 2024Article
- Effect of salinity on the oviposition and growth ofEcology and evolution · 2024Article
- Using capillary electrophoresis to identify Anopheline species in routine sampling sites.Ecology and evolution · 2024Article
- First record of the invasive mosquito species Aedes koreicus (Diptera: Culicidae) in the Czech Republic.Parasitology research · 2022Article
- First Description of the Mitogenome Features ofAnimals : an open access journal from MDPI · 2022Article
- Metagenomic Analysis ofFrontiers in cellular and infection microbiology · 2022Article
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5 authors.
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Abstract
backgroundMosquitoes, as vectors of various human pathogens, are significant drivers of serious human illness. In particular, those species in the Aedini tribe, which typically transmit dengue virus, Chikungunya fever virus, and Zika virus, are increasing their range because of climate change and international commerce. In order to evaluate the risk of disease transmission, accurate mosquito species identification and monitoring are needed. The goal of this work was to develop a rapid and simple molecular diagnostic method for six morphologically similar Aedini species (Aedes flavopictus, Aedes albopictus, Ochlerotatus koreicus, Ochlerotatus japonicus, Ochlerotatus togoi and Ochlerotatus hatorii) in Korea.
methodsA total of 109 samples were assayed in this study. The internal transcribed spacer 2 (ITS2) regions from all six species were amplified, sequenced and analyzed using Mega 6. Following the identification of regions that were consistently different in terms of sequence between all six species, multiplex primers were designed to amplify these regions to generate species-specific fragments distinguishable by their size.
resultsUniquely sized fragments were generated in Ae. flavopictus (495 bp), Ae. albopictus (438 bp), Oc. koreicus (361 bp), Oc. togoi (283 bp), Oc. hatorii (220 bp) and Oc. japonicus (160 bp). Pairwise distance analysis showed that the difference was 35.0 ± 1.5% between Aedes spp. and Ochlerotatus spp., 17.4 ± 0.2% between Ae. albopictus and Ae. flavopictus and 11.1 ± 0.3% between Oc. koreicus and Oc. japonicus.
conclusionsIn this study, a multiplex PCR assay for six species of the Aedini tribe was developed. This assay is more accurate than morphological identification and will be useful for monitoring and controlling these vector mosquitoes.
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