ArticleMicrobial cell factories2021
Fine-tuning the expression of pathway gene in yeast using a regulatory library formed by fusing a synthetic minimal promoter with different Kozak variants.
Article in Microbial cell factories, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Development of an inducible stable producer cell line for rAAV production.Biochemical engineering journal · 2026Article
- Engineering genetic elements for microbial protein expression systems: Advances, challenges, applications, and prospects.Synthetic and systems biotechnology · 2026Review
- Biological parts in yeast synthetic biology: from regulatory elements to predictive design platforms.FEMS yeast research · 2026Review
- Transcriptional regulation: efficient genetic engineering tools for non-conventional yeasts.FEMS yeast research · 2026Review
- Synthetic tunable promoters for flexible control of multi-gene expression in mammalian cells.Journal of advanced research · 2025Article
- In vivo pathway optimization in yeast via loxpsym-mediated shuffling of upstream activating sequences.Nucleic acids research · 2025Article
- Promoter engineering for enhanced 3-hydroxypropionic acid production inSynthetic and systems biotechnology · 2025Article
- Matrix regulation: a plug-and-tune method for combinatorial regulation in Saccharomyces cerevisiae.Nature communications · 2025Article
- Constitutive Promoters Functional in Plant, Fungal, and Bacterial Hosts.ACS synthetic biology · 2025Article
- Development of inducible packaging cell line for rAAV production via CRISPR-Cas9 mediated site-specific integration.Biochemical engineering journal · 2025Article
- Engineering artificial cross-species promoters with different transcriptional strengths.Synthetic and systems biotechnology · 2025Article
- Tuning fungal promoters for the expression of eukaryotic proteins.World journal of microbiology & biotechnology · 2024Review
- Single-molecule tracking reveals dynamic regulation of ribosomal scanning.Science advances · 2024Article
- Screening of broad-host expression promoters for shuttle expression vectors in non-conventional yeasts and bacteria.Microbial cell factories · 2024Article
- eIF4F complex dynamics are important for the activation of the integrated stress response.Molecular cell · 2024Article
- Utilizing 5' UTR Engineering Enables Fine-Tuning of Multiple Genes within Operons to Balance Metabolic Flux inBiology · 2024Article
- Functional analysis of the AUG initiator codon context reveals novel conserved sequences that disfavor mRNA translation in eukaryotes.Nucleic acids research · 2024Article
- A Standardized Set of MoClo-Compatible Inducible Promoter Systems for Tunable Gene Expression in Yeast.ACS synthetic biology · 2024Article
- Characterization and optimization of 5´ untranslated region containing poly-adenine tracts in Kluyveromyces marxianus using machine-learning model.Microbial cell factories · 2024Article
- Review
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Authors and funding
5 authors.
Funding
Abstract
backgroundTailoring gene expression to balance metabolic fluxes is critical for the overproduction of metabolites in yeast hosts, and its implementation requires coordinated regulation at both transcriptional and translational levels. Although synthetic minimal yeast promoters have shown many advantages compared to natural promoters, their transcriptional strength is still limited, which restricts their applications in pathway engineering.
resultsIn this work, we sought to expand the application scope of synthetic minimal yeast promoters by enhancing the corresponding translation levels using specific Kozak sequence variants. Firstly, we chose the reported UAS
conclusionsAll these results demonstrate that this chimeric promoter library developed in this study is an effective tool for pathway engineering in yeast.
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