ArticlePLoS genetics2021
Oligopaint DNA FISH reveals telomere-based meiotic pairing dynamics in the silkworm, Bombyx mori.
Article in PLoS genetics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Genome-wide crossover analysis in the holocentric pantry moth reveals strong crossover assurance and interference during spermatogenesis.Genome research · 2026Article
- Programmed meiotic errors facilitate dichotomous sperm production in the silkworm,Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Unique territorial and compartmental organization of chromosomes in the holocentric silkworm.The EMBO journal · 2026Article
- Evolution of Hybrid Inviability Associated With Chromosome Fusions.Molecular ecology · 2025Article
- OligoY pipeline for full Y chromosome painting.Briefings in bioinformatics · 2025Article
- Towards identification of a holocentromere marker in the lepidopteran model Spodoptera frugiperda.Chromosoma · 2025Article
- Whole Mount Oligopaint Fluorescence In Situ Hybridization of Mouse Oocytes, Eggs, or Early-Stage Embryos.Methods in molecular biology (Clifton, N.J.) · 2025Article
- An egg-sabotaging mechanism drives non-Mendelian transmission in mice.Current biology : CB · 2024Article
- Co-inheritance of recombined chromatids maintains heterozygosity in a parthenogenetic ant.Nature ecology & evolution · 2024Article
- Chromosome segregation during spermatogenesis occurs through a unique center-kinetic mechanism in holocentric moth species.PLoS genetics · 2024Article
- Meiotic drive against chromosome fusions in butterfly hybrids.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2024Article
- Agent-based modeling of nuclear chromosome ensembles identifies determinants of homolog pairing during meiosis.PLoS computational biology · 2024Article
- An egg sabotaging mechanism drives non-Mendelian transmission in mice.bioRxiv : the preprint server for biology · 2024Article
- Unselfish meiotic drive maintains heterozygosity in a parthenogenetic ant.bioRxiv : the preprint server for biology · 2024Article
- Translocations and inversions: major chromosomal rearrangements during Vigna (Leguminosae) evolution.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Insect multimeric G-quadruplexes fold into antiparallel structures of different compactness and stability in KRSC advances · 2023Article
- Cytogenetic screening of a Canadian swine breeding nucleus using a newly developed karyotyping method named oligo-banding.Genetics, selection, evolution : GSE · 2023Article
- Article
- Chromosome-level assembly of the Rangifer tarandus genome and validation of cervid and bovid evolution insights.BMC genomics · 2023Article
- Conserved meiotic mechanisms in the cnidarianScience advances · 2023Article
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4 authors.
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Abstract
Accurate chromosome segregation during meiosis is essential for reproductive success. Yet, many fundamental aspects of meiosis remain unclear, including the mechanisms regulating homolog pairing across species. This gap is partially due to our inability to visualize individual chromosomes during meiosis. Here, we employ Oligopaint FISH to investigate homolog pairing and compaction of meiotic chromosomes and resurrect a classical model system, the silkworm Bombyx mori. Our Oligopaint design combines multiplexed barcoding with secondary oligo labeling for high flexibility and low cost. These studies illustrate that Oligopaints are highly specific in whole-mount gonads and on meiotic squashes. We show that meiotic pairing is robust in both males and females and that pairing can occur through numerous partially paired intermediate structures. We also show that pairing in male meiosis occurs asynchronously and seemingly in a transcription-biased manner. Further, we reveal that meiotic bivalent formation in B. mori males is highly similar to bivalent formation in C. elegans, with both of these pathways ultimately resulting in the pairing of chromosome ends with non-paired ends facing the spindle pole. Additionally, microtubule recruitment in both C. elegans and B. mori is likely dependent on kinetochore proteins but independent of the centromere-specifying histone CENP-A. Finally, using super-resolution microscopy in the female germline, we show that homologous chromosomes remain associated at telomere domains in the absence of chiasma and after breakdown and modification to the synaptonemal complex in pachytene. These studies reveal novel insights into mechanisms of meiotic homolog pairing both with or without recombination.
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