Evidence map›Paper›PMID 34319449›Full record

ArticleAntonie van Leeuwenhoek2021

C-protein α-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae.

Nemanja Mirkovic, Mina Obradovic, Paula M O'Connor, Brankica Filipic, Branko Jovcic, Paul D Cotter, Milan Kojic

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Article in Antonie van Leeuwenhoek, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 3 countries.

Nemanja MirkovicLaboratory for Molecular Microbiology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444/a, 11042, Belgrade, Serbia.
Mina ObradovicLaboratory for Molecular Microbiology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444/a, 11042, Belgrade, Serbia.
Paula M O'ConnorTeagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland.
Brankica FilipicFaculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
Branko JovcicLaboratory for Molecular Microbiology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444/a, 11042, Belgrade, Serbia.
Paul D CotterTeagasc Food Research Centre, Moorepark, Fermoy, Co. Cork, Ireland.
Milan KojicLaboratory for Molecular Microbiology, Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444/a, 11042, Belgrade, Serbia. mkojic@imgge.bg.ac.rs.ORCID http://orcid.org/0000-0001-5645-750X
University of Belgrade · RSTeagasc - The Irish Agriculture and Food Development Authority · IE

Funding

Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja 173019
6 · The paper itself

Abstract

Screening for producers of potent antimicrobial peptides, resulted in the isolation of Bacillus cereus BGNM1 with strong antimicrobial activity against Listeria monocytogenes. Genome sequence analysis revealed that BGNM1 contains the gene cluster associated with the production of the lantibiotic, thusin, previously identified in B. thuringiensis. Purification of the antimicrobial activity confirmed that strain BGMN1 produces thusin. Both thusin sensitive and resistant strains were detected among clinical isolates of Streptococcus agalactiae. Random mutagenesis of a thusin sensitive strain, S. agalactiae B782, was performed in an attempt to identify the receptor protein for thusin. Three independent thusin resistant mutants were selected and their complete genomes sequenced. Comparative sequence analysis of these mutants with the WT strain revealed that duplication of a region encoding a 79 amino acids repeat in a C-protein α-antigen was a common difference, suggesting it to be responsible for increased resistance to thusin. Since induced thusin resistant mutants showed higher level of resistance than the naturally resistant B761 strain, complete genome sequencing of strain B761 was performed to check the integrity of the C-protein α-antigen-encoding gene. This analysis revealed that this gene is deleted in B761, providing further evidence that this protein promotes interaction of the thusin with receptor.

Indexed as

BacteriocinsListeria monocytogenesAnti-Bacterial AgentsMultigene FamilyStreptococcus agalactiaeAnti-Bacterial AgentsBacteriocinsAntimicrobial resistanceBacillus cereus BGNM1Bacteriocin resistanceC-protein α-antigenStreptococcus agalactiaeTwo-peptide lantibiotic thusin

Identifiers

PMID34319449
OpenAlexW3165855129

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.