ArticleAngewandte Chemie (International ed. in English)2021
Functional Switch and Ethyl Group Formation in the Bacterial Polytrichastrene Synthase from Chryseobacterium polytrichastri.
Article in Angewandte Chemie (International ed. in English), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed.
- Chemoenzymatic Synthesis of (4R,7R)-Zingiberene Enables Construction of nor-Sesquiterpene Semiochemical Analogs.Chembiochem : a European journal of chemical biology · 2026Article
- Expanding the substrate scope of a bacterial monoterpene synthase for the production of sesquiterpenoid and diterpenoid products.The FEBS journal · 2026Article
- A Single Residue Switch Controls Isomer Selectivity in the Myxobacterial Isobonnadiene Synthase.Organic letters · 2026Article
- Mechanistic Characterisation of Bacterial Terpene Synthases from Chitinophagaceae Producing Marine-Type Diterpenes.Angewandte Chemie (International ed. in English) · 2025Article
- Mechanistic Characterisation of a Diterpene Synthase for Chryseojoostenes A-E from Chryseobacterium Joostei.Angewandte Chemie (International ed. in English) · 2025Article
- Genome Mining of Terpene Synthases from FourteenMicroorganisms · 2025Article
- Mining coral-derived terpene synthases and mechanistic studies of the coral biflorane synthase.Science advances · 2025Article
- Engineering terpene synthases and their substrates for the biocatalytic production of terpene natural products and analogues.Chemical communications (Cambridge, England) · 2025Review
- Discovery of bacterial terpenoids by genome mining.Methods in enzymology · 2025Article
- Discovery of a terpene synthase synthesizing a nearly non-flexible eunicellane reveals the basis of flexibility.Nature communications · 2024Article
- Decoding Catalysis by Terpene Synthases.ACS catalysis · 2023Review
- Mechanistic Characterisation and Engineering of Sesterviolene Synthase from Streptomyces violens.Angewandte Chemie (International ed. in English) · 2023Article
- Crystal Structure Based Mutagenesis of Cattleyene Synthase Leads to the Generation of Rearranged Polycyclic Diterpenes.Angewandte Chemie (International ed. in English) · 2022Article
- Enzymatic Synthesis of Variediene Analogs.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Molecular Determinants of Carbocation Cyclisation in Bacterial Monoterpene Synthases.Chembiochem : a European journal of chemical biology · 2022Article
- Predictive Engineering of Class I Terpene Synthases Using Experimental and Computational Approaches.Chembiochem : a European journal of chemical biology · 2022Review
- Functional Switch and Ethyl Group Formation in the Bacterial Polytrichastrene Synthase from Chryseobacterium polytrichastri.Angewandte Chemie (International ed. in English) · 2021Article
- Targeting active site residues and structural anchoring positions in terpene synthases.Beilstein journal of organic chemistry · 2021Article
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3 authors.
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Abstract
A reinvestigation of the linalool synthase from Chryseobacterium polytrichastri uncovered its diterpene synthase activity, yielding polytrichastrene A and polytrichastrol A with new skeletons, besides known wanju-2,5-diene and thunbergol. The enzyme mechanism was investigated by isotopic labeling experiments and DFT calculations to explain an unusual ethyl group formation. Rationally designed exchanges of active site residues showed major functional switches, resulting for I66F in the production of five more new compounds, including polytrichastrene B and polytrichastrol B, while A87T, A192V and the double exchange A87T, A192V gave a product shift towards wanju-2,5-diene.
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