Evidence map›Paper›PMID 34318335›Full record

ArticleAnalytical and bioanalytical chemistry2021

Enzymatic electrochemical biosensor for glyphosate detection based on acid phosphatase inhibition.

Preeyanut Butmee, Gamolwan Tumcharern, Chomphunuch Songsiriritthigul, Marie José Durand, Gerald Thouand, Margaret Kerr, Kurt Kalcher, Anchalee Samphao

Abstract read
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In one paragraph

Article in Analytical and bioanalytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.5field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 25 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 8 institutions in 4 countries.

Preeyanut ButmeeNational Nanotechnology Center, National Science and Technology Development Agency, Pathumthani, 12120, Thailand.
Gamolwan TumcharernNational Nanotechnology Center, National Science and Technology Development Agency, Pathumthani, 12120, Thailand. gamolwan@nanotec.or.th.
Chomphunuch SongsiriritthigulSynchrotron Light Research Institute (Public Organization), 111 University Avenue, Nakhon Ratchasima, 30000, Thailand.
Marie José DurandNantes Université, ONIRIS, CNRS, GEPEA, UMR 6144, F-85000, La Roche sur Yon, France.
Gerald ThouandNantes Université, ONIRIS, CNRS, GEPEA, UMR 6144, F-85000, La Roche sur Yon, France.
Margaret KerrDepartment of Chemistry, Worcester State University, 486 Chandler Street, Worcester, MA, 01602, United States.
Kurt KalcherInstitute of Chemistry-Analytical Chemistry, University of Graz, A-8010, Graz, Austria.
Anchalee SamphaoDepartment of Chemistry, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani, 34190, Thailand. anchalee.s@ubu.ac.th.
Centre National de la Recherche Scientifique · FRNantes Université · FRNational Nanotechnology Center · THNational Science and Technology Development Agency · THSynchrotron Light Research Institute · THUbon Ratchathani University · THUniversity of Graz · ATWorcester State University · US

Funding

Thailand Graduate Institute of Science and Technology TGIST grant No. TG-55-24-60-010D
6 · The paper itself

Abstract

A novel enzymatic electrochemical biosensor was fabricated for the indirect detection of glyphosate-based acid phosphatase inhibition. The biosensor was constructed on a screen-printed carbon electrode modified with silver nanoparticles, decorated with electrochemically reduced graphene oxide, and chemically immobilized with acid phosphatase via glutaraldehyde cross-linking. We measured the oxidation current by chronoamperometry. The current arose from the enzymatic reaction of acid phosphatase and the enzyme-substrate disodium phenyl phosphate. The biosensing response is a decrease in signal resulting from inhibition of acid phosphatase in the presence of glyphosate inhibitor. The inhibition of acid phosphatase by glyphosate was investigated as a reversible competitive-type reaction based on the Lineweaver-Burk equation. Computational docking confirmed that glyphosate was the inhibitor bound in the substrate-binding pocket of acid phosphatase and that it was able to inhibit the enzyme efficiently. Additionally, the established method was applied to the selective analysis of glyphosate in actual samples with satisfactory results following a standard method.

Indexed as

Acid PhosphataseBiosensing TechniquesElectrochemical TechniquesEnzymes, ImmobilizedGlycineGlyphosateHerbicidesKineticsLimit of DetectionMolecular Docking SimulationReproducibility of ResultsSpectrum Analysis, RamanAcid PhosphataseEnzymes, ImmobilizedGlycineGlyphosateHerbicidesAcid phosphataseElectrochemical biosensorGlyphosateGraphene oxideSilver nanoparticles

Identifiers

PMID34318335
OpenAlexW3184130928

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.