Evidence map›Paper›PMID 34316219›Full record

ArticleVeterinary world2021

Expression of recombinant 35 kDa fragment of VP2 protein of canine parvovirus using

Natnaree Inthong, Sarawan Kaewmongkol, Nattakan Meekhanon, Eukote Suwan, Wanat Sricharern, Khomson Satchasataporn, Rungthiwa Sinsiri, Kaitkanoke Sirinarumitr, Theerapol Sirinarumitr

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Article in Veterinary world, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Natnaree InthongCenter for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
Sarawan KaewmongkolDepartment of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand.
Nattakan MeekhanonDepartment of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand.
Eukote SuwanDepartment of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand.
Wanat SricharernDepartment of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand.
Khomson SatchasatapornDepartment of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand.
Rungthiwa SinsiriMolecular Diagnostic Laboratory, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
Kaitkanoke SirinarumitrCenter of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok 10900, Thailand.
Theerapol SirinarumitrCenter of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok 10900, Thailand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

aimCanine parvovirus (CPV) is one of the most common viral infections in dogs, causing acute hemorrhagic gastroenteritis and high mortality. Vaccination effectively prevents CPV infection. However, the currently available CPV vaccines have concerns such as maternal immunity interference, shedding of virus vaccine, and false-positive result based on polymerase chain reaction after vaccination. A subunit vaccine can overcome these problems. This study aimed to express the recombinant 35 kDa fragment of the VP2 protein (consisting of epitopes 1-7) and the recombinant full-length VP2 protein (consisting of epitopes 1-10) and to study the ability of these two recombinant proteins to react with rabbit anti-CPV polyclonal antibodies. MATERIALS AND

methodsThe full length and 35 kDa fragment of

resultsThe recombinant full-length and the recombinant 35 kDa fragment

conclusionThese results suggest that the recombinant 35 kDa fragment and the recombinant full-length VP2 proteins may be useful in developing a CPV diagnostic test or vaccine.

Indexed as

canine parvovirusesEscherichia coli expression systemrecombinant proteinVP2 gene

Identifiers

PMID34316219
PMCPMC8304427

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