ArticleNature communications2021
Correlative 3D microscopy of single cells using super-resolution and scanning ion-conductance microscopy.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- SPIFFI enables single-shot super-resolution and multidimensional imaging.Nature methods · 2026Article
- Perspective on Scanning Electrochemical Probe Microscopy in Single-Cell Imaging.Chemical & biomedical imaging · 2026Review
- Live-Cell SICM Imaging: An Introductory Guide for New Users.Analytical chemistry · 2026Article
- A Correlative SICM-OPM Platform for Surface and Volumetric Imaging in Live Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Applications of Nanopipettes in Scanning Ion Conductance Microscopy for High-Spatial-Resolution Topographic Imaging and Sensing in Single Cells.ACS measurement science au · 2026Review
- Revealing the MRI-Contrast in Optically Cleared Brains.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Using adjusted local assortativity with Molecular Pixelation unveils colocalization of membrane proteins with immunological significance.Frontiers in immunology · 2024Article
- Precision Delivery Using Nanopipette for Single-cell Studies.Dian hua xue · 2024Article
- Live-Cell SOFI Correlation with SMLM and AFM Imaging.ACS bio & med chem Au · 2023Article
- Charge Mapping ofAnalytical chemistry · 2023Article
- Review
- The New Era of High-Throughput Nanoelectrochemistry.Analytical chemistry · 2023Review
- Photochemical Mechanisms of Fluorophores Employed in Single-Molecule Localization Microscopy.Angewandte Chemie (International ed. in English) · 2023Review
- A OneStep Solution to Fix and Stain Cells for Correlative Live and Fixed Microscopy.Current protocols · 2021Article
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-resolution live-cell imaging is necessary to study complex biological phenomena. Modern fluorescence microscopy methods are increasingly combined with complementary, label-free techniques to put the fluorescence information into the cellular context. The most common high-resolution imaging approaches used in combination with fluorescence imaging are electron microscopy and atomic-force microscopy (AFM), originally developed for solid-state material characterization. AFM routinely resolves atomic steps, however on soft biological samples, the forces between the tip and the sample deform the fragile membrane, thereby distorting the otherwise high axial resolution of the technique. Here we present scanning ion-conductance microscopy (SICM) as an alternative approach for topographical imaging of soft biological samples, preserving high axial resolution on cells. SICM is complemented with live-cell compatible super-resolution optical fluctuation imaging (SOFI). To demonstrate the capabilities of our method we show correlative 3D cellular maps with SOFI implementation in both 2D and 3D with self-blinking dyes for two-color high-order SOFI imaging. Finally, we employ correlative SICM/SOFI microscopy for visualizing actin dynamics in live COS-7 cells with subdiffraction-resolution.
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