ArticleMethods in molecular biology (Clifton, N.J.)2021
Giardia duodenalis: Detection by Quantitative Real-Time PCR and Molecular Diversity.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
3 citing papers in PubMed, 1 synthesis or guideline pooled it, 4 citations in OpenAlex.
- Prevalence and genotyping of Giardia duodenalis infections in humans in Thailand: a systematic review and meta-analysis.BMC infectious diseases · 2025Pooled it
- Mucosal vaccination in a murine gnotobiotic model ofInfection and immunity · 2024Article
- Burden and Epidemiology of Human IntestinalTropical medicine and infectious disease · 2022Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conventional microscopy is being progressively replaced by molecular (PCR and Sanger sequencing) methods for the first-line detection, identification, and genotyping of diarrhea-causing enteric parasites in modern clinical and research laboratories. These technologies allow increased sample testing, improved throughput, and optimized laboratory workflow in a cost-effective manner. Here, we describe the methodological algorithm used in a national reference center for the diagnosis and molecular characterization of Giardia duodenalis, a major contributor to the enormous burden of diarrheal disease globally. Initial detection of the parasite is conducted by a sensitive real-time PCR to amplify the small subunit of the ribosomal RNA of G. duodenalis, whereas a multilocus sequence genotyping scheme based on three constitutive genetic markers (glutamate dehydrogenase, β-giardin, and triose phosphate isomerase) is used for genotyping and sub-genotyping purposes. The advantages and disadvantages of these PCR-based methods are commented.
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