Evidence map›Paper›PMID 34313984›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

An Illumina MiSeq-Based Amplicon Sequencing Method for the Detection of Mixed Parasite Infections Using the Blastocystis SSU rRNA Gene as an Example.

Jenny G Maloney, Nadja S George, Aleksey Molokin, Monica Santin

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Genetic diversity of single-celled microorganismCurrent research in microbial sciences · 2025
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Jenny G MaloneyUnited States Department of Agriculture, Agricultural Research Service, Environmental Microbial Food Safety Laboratory, Beltsville, MD, USA. jenny.maloney@usda.gov.
Nadja S GeorgeUnited States Department of Agriculture, Agricultural Research Service, Environmental Microbial Food Safety Laboratory, Beltsville, MD, USA.
Aleksey MolokinUnited States Department of Agriculture, Agricultural Research Service, Environmental Microbial Food Safety Laboratory, Beltsville, MD, USA.
Monica SantinUnited States Department of Agriculture, Agricultural Research Service, Environmental Microbial Food Safety Laboratory, Beltsville, MD, USA.
Agricultural Research Service · USUnited States Department of Agriculture · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Parasite mixed infections remain a relatively unexplored field in part due to the difficulties of unraveling complex mixtures of parasite DNA using classical methods of sequencing. Next-generation amplicon sequencing (NGS) is a powerful tool for exploring mixed infections of multiple genetic variants of the same parasite in clinical, environmental (water or soil), or food samples. Here, we provide a method for NGS-based detection of mixed parasite infections which uses the Blastocystis SSU rRNA gene as an example and includes steps for parasite concentration, DNA extraction, sequencing library preparation, and bioinformatic analysis.

Indexed as

BlastocystisCoinfectionParasitic DiseasesAnimalsGenes, rRNAHigh-Throughput Nucleotide SequencingBlastocystisIllumina MiseqMixed infectionsNext-generation amplicon sequencingNGSParasitesProtistSubtypes

Identifiers

PMID34313984
OpenAlexW3185764604

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.