ArticleNucleic acids research2021
Visualization and characterization of RNA-protein interactions in living cells.
Article in Nucleic acids research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- Article
- Review
- TET dioxygenases localize at splicing speckles and promote RNA splicing.Nucleus (Austin, Tex.) · 2025Article
- Single-protein/RNA imaging reveals ZNF598 as a limiting factor in resolving collided ribosomes.The EMBO journal · 2025Article
- BioLLMNet: enhancing RNA-interaction prediction with a specialized cross-LLM transformation network.Briefings in bioinformatics · 2025Article
- CRISPR RiPCA for Investigating eIF4E-mACS chemical biology · 2025Article
- Long non-coding RNAs: roles in cellular stress responses and epigenetic mechanisms regulating chromatin.Nucleus (Austin, Tex.) · 2024Review
- A task-specific encoding algorithm for RNAs and RNA-associated interactions based on convolutional autoencoder.Nucleic acids research · 2023Article
- STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity.Nature communications · 2022Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA-protein interactions are the structural and functional basis of significant numbers of RNA molecules. RNA-protein interaction assays though, still mainly depend on biochemical tests in vitro. Here, we establish a convenient and reliable RNA fluorescent three-hybrid (rF3H) method to detect/interrogate the interactions between RNAs and proteins in cells. A GFP tagged highly specific RNA trap is constructed to anchor the RNA of interest to an artificial or natural subcellular structure, and RNA-protein interactions can be detected and visualized by the enrichment of RNA binding proteins (RBPs) at these structures. Different RNA trapping systems are developed and detection of RNA-protein complexes at multiple subcellular structures are assayed. With this new toolset, interactions between proteins and mRNA or noncoding RNAs are characterized, including the interaction between a long noncoding RNA and an epigenetic modulator. Our approach provides a flexible and reliable method for the characterization of RNA-protein interactions in living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.