ArticleThe Plant journal : for cell and molecular biology2021
Overexpression of a wheat α-amylase type 2 impact on starch metabolism and abscisic acid sensitivity during grain germination.
Article in The Plant journal : for cell and molecular biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 20 citations in OpenAlex.
- Soybean α-Amylase Gene Family: Structure and Expression in Response to Abiotic Stresses.International journal of molecular sciences · 2026Article
- Obesity exacerbates experimental colitis due to the activation of the local renin-angiotensin system in the colon.Scientific reports · 2025Article
- Genome-wide identification of the AcBAM family in kiwifruit (Actinidia chinensis cv. Hongyang) and the expression profiling analysis of AcBAMs reveal their role in starch metabolism.BMC plant biology · 2025Article
- Comparative miRNAome combined with transcriptome and degradome analysis reveals a novel miRNA-mRNA regulatory network associated with starch metabolism affecting pre-harvest sprouting resistance in wheat.BMC plant biology · 2025Article
- Molecular Characteristics and Functional Identification of a Key Alpha-Amylase-Encoding GeneInternational journal of molecular sciences · 2024Article
- Effect of Different Wheat Sprouting Conditions on the Characteristics of Whole-Wheat Flour.Food technology and biotechnology · 2024Article
- A mediator of OsbZIP46 deactivation and degradation negatively regulates seed dormancy in rice.Nature communications · 2024Article
- RNA-seq study reveals the signaling and carbohydrate metabolism regulators involved in dormancy release by warm stratification inPlant biotechnology (Tokyo, Japan) · 2022Article
- Dynamic RNA-Seq Study Reveals the Potential Regulators of Seed Germination inPlants (Basel, Switzerland) · 2022Article
- A banana transcriptional repressor MaAP2a participates in fruit starch degradation during postharvest ripening.Frontiers in plant science · 2022Article
- Over-Expression of a Wheat Late Maturity Alpha-Amylase Type 1 Impact on Starch Properties During Grain Development and Germination.Frontiers in plant science · 2022Article
- Physiological, Transcriptional, and Metabolic Variations During Dormancy Release Across Potato Cultivars Revealed by Integrated Multi-Omics Analysis.Physiologia plantarumArticle
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite being of vital importance for seed establishment and grain quality, starch degradation remains poorly understood in organs such as cereal or legume seeds. In cereals, starch degradation requires the synergetic action of different isoforms of α-amylases. Ubiquitous overexpression of TaAmy2 resulted in a 2.0-437.6-fold increase of total α-amylase activity in developing leaf and harvested grains. These increases led to dramatic alterations of starch visco-properties and augmentation of soluble carbohydrate levels (mainly sucrose and α-gluco-oligosaccharide) in grain. Interestingly, the overexpression of TaAMY2 led to an absence of dormancy in ripened grain due to abscisic acid (ABA) insensitivity. Using an allosteric α-amylase inhibitor (acarbose), we demonstrated that ABA insensitivity was due to the increased soluble carbohydrate generated by the α-amylase excess. Independent from the TaAMY2 overexpression, inhibition of α-amylase during germination led to the accumulation of soluble α-gluco-oligosaccharides without affecting the first stage of germination. These findings support the hypotheses that (i) endosperm sugar may overcome ABA signalling and promote sprouting, and (ii) α-amylase may not be required for the initial stage of grain germination, an observation that questions the function of the amylolytic enzyme in the starch degradation process during germination.
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