ArticleApplied biochemistry and biotechnology2021
Metabolic Engineering of Central Carbon Metabolism of Bacillus licheniformis for Enhanced Production of Poly-γ-glutamic Acid.
Article in Applied biochemistry and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Metabolic Engineering ofMicroorganisms · 2025Article
- Overflow metabolism in bacterial, yeast, and mammalian cells: different names, same game.Molecular systems biology · 2025Review
- Overexpressing Endopeptidase Inhibitor IseA Enhances Biomass and Biochemical Production of Bacillus licheniformis.Current microbiology · 2025Article
- Multilevel metabolic engineering for enhanced synthesis of S-adenosylmethionine by Bacillus amyloliquefaciens.Biotechnology letters · 2024Article
- Enhanced Poly-γ-Glutamic Acid Production by a Newly Isolated Bacillus halotolerans F29.Journal of microbiology (Seoul, Korea) · 2024Article
- Genomic characterization and related functional genes of γ- poly glutamic acid producing Bacillus subtilis.BMC microbiology · 2024Article
- Synthesis of Poly-γ-Glutamic Acid and Its Application in Biomedical Materials.Materials (Basel, Switzerland) · 2023Review
- Advances in the optimization of central carbon metabolism in metabolic engineering.Microbial cell factories · 2023Review
- Engineering of a newly isolated Bacillus tequilensis BL01 for poly-γ-glutamic acid production from citric acid.Microbial cell factories · 2022Article
- Genetic and metabolic engineering for poly-γ-glutamic acid production: current progress, challenges, and prospects.World journal of microbiology & biotechnology · 2022Review
- γ-Polyglutamic Acid Production, Biocontrol, and Stress Tolerance: Multifunction ofInternational journal of environmental research and public health · 2022Article
- Engineering the glyoxylate cycle for chemical bioproduction.Frontiers in bioengineering and biotechnology · 2022Review
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Poly-γ-glutamic acid (γ-PGA) is an anionic polymer with wide-ranging applications in the areas of medicine, light chemical industry, wastewater treatment, and agriculture. However, the production cost of γ-PGA is high for the requirement of adding the expensive precursor L-glutamic acid during fermentation, which hinders its widespread application. In this study, in order to improve γ-PGA yield, central carbon metabolism was engineered to enhance the carbon flux of tricarboxylic acid (TCA) cycle and glutamic acid synthesis in a γ-PGA production strain Bacillus licheniformis WX-02. Firstly, pyruvate dehydrogenase (PdhABCD) and citrate synthase (CitA) were overexpressed to strengthen the flux of pyruvate into TCA cycle, resulting in 34.93% and 11.14% increase of γ-PGA yield in B. licheniformis WX-02, respectively. Secondly, the carbon flux to glyoxylate shunt was rewired via varying the expression of isocitrate lyase (AceA), and a 23.24% increase of γ-PGA yield was obtained in AceA down-regulated strain WXP
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