ArticleNature communications2021
BiPOLES is an optogenetic tool developed for bidirectional dual-color control of neurons.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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70 citing papers in PubMed.
- All-optical closed-loop control of human cardiomyocyte networks exploiting holographic optogenetics.Communications engineering · 2026Article
- Crosstalk-free optical indicator-actuator combinations for bidirectional optogenetics.npj biomedical innovations · 2026Review
- Neuropixels Opto: combining high-resolution electrophysiology and optogenetics.Nature methods · 2026Article
- Theoretical analysis of low power synergistic sono-optogenetic control of calcium-dependent synaptic plasticity.Scientific reports · 2026Article
- Projection-specific intersectional optogenetics for precise excitation and inhibition in the marmoset brain.Cell reports methods · 2026Article
- Blood-catalyzed n-doped polymers for reversible optical neural control.Science (New York, N.Y.) · 2026Article
- Neural substrate of conditioned stimulus for associative learning in the hippocampus.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Bridging Model and Experiment in Systems Neuroscience with Cleo: The Closed-Loop, Electrophysiology, and Optophysiology Simulation Testbed.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- Multicolor photoreactions of the red light-activated channelrhodopsin Chrimson.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Oxytocin modulates respiratory heart rate variability through a hypothalamus-brainstem-heart neuronal pathway.Nature neuroscience · 2025Article
- Interneuron theta phase locking controls seizure susceptibility.bioRxiv : the preprint server for biology · 2025Article
- Two Decades of Optogenetic Tools: A Retrospective and a Look Ahead.Advanced genetics (Hoboken, N.J.) · 2025Review
- Bridging model and experiment in systems neuroscience with Cleo: the Closed-Loop, Electrophysiology, and Optophysiology simulation testbed.bioRxiv : the preprint server for biology · 2025Article
- Delay suppression control of β-oscillations: a proposal for dual-target adaptive deep brain stimulation on STN-GPe network model.Biological cybernetics · 2025Article
- CryoRhodopsins: A comprehensive characterization of a group of microbial rhodopsins from cold environments.Science advances · 2025Article
- Design considerations for optogenetic applications of soft micro-LED-based device systems across diverse nervous systems.Bioactive materials · 2025Review
- Article
- Optogenetic engineering for ion channel modulation.Current opinion in chemical biology · 2025Review
- Foundry-fabricated dual-color nanophotonic neural probes for photostimulation and electrophysiological recording.Neurophotonics · 2025Article
10 more citing papers are in PubMed but not listed here.
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20 authors.
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Abstract
Optogenetic manipulation of neuronal activity through excitatory and inhibitory opsins has become an indispensable experimental strategy in neuroscience research. For many applications bidirectional control of neuronal activity allowing both excitation and inhibition of the same neurons in a single experiment is desired. This requires low spectral overlap between the excitatory and inhibitory opsin, matched photocurrent amplitudes and a fixed expression ratio. Moreover, independent activation of two distinct neuronal populations with different optogenetic actuators is still challenging due to blue-light sensitivity of all opsins. Here we report BiPOLES, an optogenetic tool for potent neuronal excitation and inhibition with light of two different wavelengths. BiPOLES enables sensitive, reliable dual-color neuronal spiking and silencing with single- or two-photon excitation, optical tuning of the membrane voltage, and independent optogenetic control of two neuronal populations using a second, blue-light sensitive opsin. The utility of BiPOLES is demonstrated in worms, flies, mice and ferrets.
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