ArticleBMC veterinary research2021
Comparative iTRAQ proteomics identified proteins associated with sperm maturation between yak and cattleyak epididymis.
Article in BMC veterinary research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 15 citations in OpenAlex.
- Proteomic signatures of cervical mucus associated with fertility in Bali heifers (Veterinary world · 2026Article
- Article
- Transcriptome and Metabolome Analyses Reveal High-Altitude Adaptation Mechanism of Epididymis Sperm Maturation in Tibetan Sheep.Animals : an open access journal from MDPI · 2024Article
- Proteome Profiling of Canine Epididymal Fluid: In Search of Protein Markers of Epididymal Sperm Motility.International journal of molecular sciences · 2023Article
- iTRAQ-based comparative proteomics reveal an enhancing role of PRDX6 in the freezability of Mediterranean buffalo sperm.BMC genomics · 2023Article
- The Biological Characteristics and Differential Expression Patterns ofAnimals : an open access journal from MDPI · 2023Article
- Identification and analysis of differentially expressed (DE) circRNA in epididymis of yak and cattleyak.Frontiers in veterinary science · 2023Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundDuring maturation, spermatozoa acquire motility and fertilizing capacity as they transit through the epididymis. In recent years, two-dimensional gel electrophoresis has been employed in proteomics studies conducted in rat, boar and human. However, there has not been a complete information regarding the proteins associated with sperm maturation in the epididymis. In this study, we employed iTRAQ proteomics to investigate proteins associated with sperm maturation between yak and cattleyak epididymis.
resultsAfter a successful sampling and protein extraction, the iTRAQ coupled with LC-MS/MS mass spectrometry and bioinformatics analysis were performed. We identified 288 differentially abundant proteins (DAPs) between yak and cattleyak epididymis; 151 were up-regulated while 137 were down-regulated in cattleyak relative to yak. Gene Ontology analysis identified that down-regulated DAPs in cattleyak were mostly enriched in the acetylation of protein component, along with negative and positive regulatory activities. iTRAQ proteomics data showed that the top up-regulated DAPs were mainly enriched in cell communication, cell adhesion, cytoskeleton organization, stress response, post-translational modifications and metabolic functions while the down-regulated DAPs were predominantly associated with sperm maturation, long-term sperm storage, sperm forward motility, sperm-oocyte fusion and regulatory functions.
conclusionThese results provide insight into the molecular mechanisms underlying male cattleyak sterility.
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