Evidence map›Paper›PMID 34311349›Full record

ArticleThe Science of the total environment2021

MicroRNA-based host response to toxicant exposure is influenced by the presence of gut microbial populations.

Maggie R Williams, Robert D Stedtfeld, Tiffany M Stedtfeld, Robert B Crawford, Tomomi Kuwahara, Norbert E Kaminski, James M Tiedje, Syed A Hashsham

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Science of the total environment, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 51% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Maggie R WilliamsSchool of Engineering & Technology, Institute for Great Lakes Research, Central Michigan University, Mt Pleasant, MI, USA.
Robert D StedtfeldSwift Biosciences, Ann Arbor, MI, USA.
Tiffany M StedtfeldSwift Biosciences, Ann Arbor, MI, USA.
Robert B CrawfordInstitute for Integrative Toxicology, Michigan State University, East Lansing, MI 48824, USA; Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824, USA.
Tomomi KuwaharaDepartment of Microbiology, Faculty of Medicine, Kagawa University, Kagawa, Japan.
Norbert E KaminskiInstitute for Integrative Toxicology, Michigan State University, East Lansing, MI 48824, USA; Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI 48824, USA; Center for Research on Ingredient Safety, Michigan State University, East Lansing, MI 48824, USA.
James M TiedjeCenter for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA; Department of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, MI 48824, USA.
Syed A HashshamCenter for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA; Department of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, MI 48824, USA; Department of Civil and Environmental Engineering, East Lansing, MI 48824, USA. Electronic address: hashsham@egr.msu.edu.
Michigan State University · USSwift Engineering (United States) · USCentral Michigan University · USKagawa University · JP

Funding

VOLATILE ORGANIC CONTAMINANTS--QUANTIFYING THEIR MOVEMENT IN THE UNSATURATED ZONEP42ES004911 · NIEHS · MICHIGAN STATE UNIVERSITY · PI Jamie J Bernard · 1989 to 2026
$64.9M
NIEHS NIH HHS P42 ES004911
6 · The paper itself

Abstract

Segmented filamentous bacteria (SFB) and Bacteroides fragilis are known to interact with the host immune response through the aryl hydrocarbon receptor (Ahr). 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), an environmental toxicant and a high-affinity Ahr ligand has the potential to modify the effect of SFB and B. fragilis. MicroRNAs (miRNA) with their role in regulating gene expression post-transcriptionally, may potentially be used to observe such interactions between SFB, B. fragilis, and TCDD. However, little is known regarding the impact of gut microbial members on miRNA expression or its modulation in the presence of an environmental toxicant. This information is important in understanding toxicant-mediated dysbiosis in gut microbiome and the resulting human health impacts. In this study, C57BL/6 germ-free (GF) mice were colonized with SFB and B. fragilis and administered 30 μg/kg TCDD every 4 d for 28 d and miRNA were measured. Compared to GF mice, colonization with SFB resulted in an increase in up- and down-regulated Ileal miRNAs. TCDD treatment of this group decreased the number of upregulated miRNA and increased the number of down-regulated miRNAs. Association with SFB and B. fragilis together had a similar but less pronounced effect in response to TCDD treatment. TCDD treatment of GF mice had no miRNA expression response. Immune and inflammatory responses and T-cell differentiation were the key functions impacted by these miRNAs. Overall, these results reveal that the host response to toxicants may also depend on the presence of specific gut microbial populations.

Indexed as

Gastrointestinal MicrobiomeMicroRNAsPolychlorinated DibenzodioxinsAnimalsImmunityMiceMice, Inbred C57BLReceptors, Aryl HydrocarbonMicroRNAsPolychlorinated DibenzodioxinsReceptors, Aryl HydrocarbonGut microbiomemicroRNAsNanoString nCounterSegmented filamentous bacteriaTCDD

Identifiers

PMID34311349
PMCPMC8464502
OpenAlexW3185054175

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.