Evidence map›Paper›PMID 34309402›Full record

ArticleJournal of bacteriology2021

A Rationally Designed c-di-AMP Förster Resonance Energy Transfer Biosensor To Monitor Nucleotide Dynamics.

Alex J Pollock, Philip H Choi, Shivam A Zaver, Liang Tong, Joshua J Woodward

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of bacteriology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Alex J PollockDepartment of Microbiology, University of Washington, Seattle, Washington, USA.
Philip H ChoiDepartment of Biological Sciences, Columbia University, New York, New York, USA.
Shivam A ZaverDepartment of Microbiology, University of Washington, Seattle, Washington, USA.
Liang TongDepartment of Biological Sciences, Columbia University, New York, New York, USA.
Joshua J WoodwardDepartment of Microbiology, University of Washington, Seattle, Washington, USA.

Funding

TRAINING IN MOLECULAR AND CELLULAR BIOLOGYT32GM007270 · NIGMS · UNIVERSITY OF WASHINGTON · PI RAIBLE, DAVID W · 1985 to 2020
$21.0M
Listeria monocytogenes physiology and host pathogen interactionsR01AI116669 · NIAID · UNIVERSITY OF WASHINGTON · PI WOODWARD, JOSHUA · 2015 to 2024
$4.4M
FRET based imaging of cyclic dinucleotide dynamics in living systemsR21AI153820 · NIAID · UNIVERSITY OF WASHINGTON · PI WOODWARD, JOSHUA · 2020 to 2021
$459k
Signal transduction of cGAMP during innate immune responsesR21AI137758 · NIAID · UNIVERSITY OF WASHINGTON · PI WOODWARD, JOSHUA · 2018 to 2019
$429k
NIAID NIH HHS R01 AI116669NIAID NIH HHS R21 AI137758NIAID NIH HHS R21 AI153820NIGMS NIH HHS T32 GM007270NIH HHS 1R21AI153820-01NIH HHS AI116669NIH HHS T32GM007270
6 · The paper itself

Abstract

3'3'-Cyclic di-AMP (c-di-AMP) is an important nucleotide second messenger found throughout the bacterial domain of life. c-di-AMP is essential in many bacteria and regulates a diverse array of effector proteins controlling pathogenesis, cell wall homeostasis, osmoregulation, and central metabolism. Despite the ubiquity and importance of c-di-AMP, methods to detect this signaling molecule are limited, particularly at single-cell resolution. In this work, crystallization of the Listeria monocytogenes c-di-AMP effector protein Lmo0553 enabled structure-guided design of a Förster resonance energy transfer (FRET)-based biosensor, which we have named CDA5. CDA5 is a fully genetically encodable, specific, and reversible biosensor which allows the detection of c-di-AMP dynamics both

Indexed as

Biosensing TechniquesFluorescence Resonance Energy TransferBacillus subtilisBacterial ProteinsDinucleoside PhosphatesGene Expression Regulation, BacterialListeria monocytogenesModels, MolecularMutationNucleotidesProtein ConformationBacterial Proteinscyclic diadenosine phosphateDinucleoside PhosphatesNucleotidesbiosensorc-di-AMPcrystal structureDRaCALAFACSFRETlmo0553protein engineeringsingle cell

Identifiers

PMID34309402
PMCPMC8425405

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.