ArticleMicrobial biotechnology2021
A newly developed PCR-based method revealed distinct Fusobacterium nucleatum subspecies infection patterns in colorectal cancer.
Article in Microbial biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- Global prevalence of Fusobacterium nucleatum and Bacteroides fragilis in patients with colorectal cancer: an overview of case reports/case series and meta-analysis of prevalence studies.BMC gastroenterology · 2025Pooled it
- Full-length 16S rRNA nanopore sequencing enables species resolution ofGut microbes · 2026Article
- Integrating ANI and phylogenies for re-evaluation of Fusobacterium taxonomy and disease associations.Nature communications · 2026Article
- From gut to mouth: salivary signatures of carcinogenic microbiota.Frontiers in cellular and infection microbiology · 2026Review
- Taxonomy, carcinogenic mechanisms, and advanced molecular diagnosis of Fusobacterium nucleatum in colorectal cancer: from bench to clinical practice.Frontiers in immunology · 2026Review
- A Modified Method Incorporating Multiplex PCR Reveals Fusobacterium Prevalence in Southern Chinese Population and Its Correlations in Cancers.Microbial biotechnology · 2025Article
- Fusobacterium lineage profiling facilitates the clarification of the associations between non-nucleatum Fusobacterium and colorectal cancer.NPJ biofilms and microbiomes · 2025Article
- Fusobacterium nucleatum is enriched in invasive biofilms in colorectal cancer.NPJ biofilms and microbiomes · 2025Article
- Review
- The rise and evolving role ofCurrent research in microbial sciences · 2025Review
- Association ofGut microbiome (Cambridge, England) · 2025Review
- Microbial manipulators:Journal of oral microbiology · 2025Review
- The Involvement of Oral Pathogenic Bacteria,Gastro hep advances · 2025Article
- Optimizing Red Light-Based Photodynamic Therapy for Effective Bactericidal Action AgainstPathogens (Basel, Switzerland) · 2024Article
- Parvimonas micra forms a distinct bacterial network with oral pathobionts in colorectal cancer patients.Journal of translational medicine · 2024Article
- Circulating Bacterial DNA in Colorectal Cancer Patients: The Potential Role ofInternational journal of molecular sciences · 2024Article
- Application ofWorld journal of gastrointestinal oncology · 2024Article
- Stratification of Fusobacterium nucleatum by local health status in the oral cavity defines its subspecies disease association.Cell host & microbe · 2024Article
- Oral Microbial Profile Analysis in Patients with Oral and Pharyngeal Cancer Reveals That TumoralMicroorganisms · 2023Article
- Gut Microbial and Associated Metabolite Markers for Colorectal Cancer Diagnosis.Microorganisms · 2023Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fusobacterium nucleatum, which has four subspecies (nucleatum, animalis, vincentii and polymorphum), plays an important role in promoting colorectal cancer (CRC). However, as there is no efficient method of differentiating these subspecies in the context of a rich gut microbiota, the compositions in CRC remain largely unknown. In this study, a PCR-based differentiation method enabling profiling of F. nucleatum infection in CRC at the subspecies level was developed. Based on the analysis of 53 F. nucleatum genomes, we identified genetic markers specific to each subspecies and designed primers for the conserved sequences of those markers. The PCR performance of the primers was tested with F. nucleatum and non-nucleatum Fusobacterium strains, and complete consistence with taxonomy was achieved. Additionally, no non-specific amplification occurred when using human DNA. The method was then applied to faecal (n = 58) and fresh-frozen tumour tissue (n = 100) samples from CRC patients, and wide heterogeneity in F. nucleatum subspecies compositions in the gut microbiota among CRC patients was observed. Single-subspecies colonization was common, whereas coexistence of four subspecies was rare. Subspecies animalis was most prevalent, while nucleatum was not frequently detected. The results of this study contribute to our understanding of the pathogenicity of F. nucleatum at the subspecies level and the method developed has potential for clinical and epidemiological use.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.