Evidence map›Paper›PMID 34308964›Full record

ArticleBioscience reports2021

Microarray analysis of genes with differential expression of m6A methylation in lung cancer.

Shuo Wu, Xing Lv, Yan Zhang, Xi Xu, Feng Zhao, Yao Zhang, Lizhan Chen, Haifeng Ou-Yang, Xinyu Ti

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioscience reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Shuo Wu *Department of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, Shanxi 710032, China.
Xing Lv *Department of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, Shanxi 710032, China.
Yan ZhangDepartment of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, Shanxi 710032, China.
Xi XuDepartment of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, Shanxi 710032, China.
Feng ZhaoDepartment of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, Shanxi 710032, China.
Yao ZhangDepartment of Pulmonary Medicine, Thoracis Hospital Xi'an International Medical Center, Xi'an, Shanxi 710100, China.
Lizhan ChenDepartment of Pulmonary Medicine, Thoracis Hospital Xi'an International Medical Center, Xi'an, Shanxi 710100, China.
Haifeng Ou-YangDepartment of Pulmonary Medicine, Thoracis Hospital Xi'an International Medical Center, Xi'an, Shanxi 710100, China.
Xinyu TiDepartment of Pulmonary and Critical Care Medicine, Xijing Hospital, Fourth Military Medical University, Xi'an, Shanxi 710032, China.ORCID 0000-0001-8578-1368
Xijing Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeN6-methyladenosine (m6A) is among the most abundant mRNA modifications in eukaryote. The aim of the present study was to investigate function of m6A mRNA methylation in lung cancer and the underlying mechanism.

methodsMicroarray analysis was performed to detect the differences in RNA expression between cancerous and adjacent non-cancerous tissue samples. The target mRNAs were subjected to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. Hierarchical clustering of RNAs was conducted to identify distinct m6A methylation or expression patterns between the samples.

resultsIn the present study, some differentially expressed genes (DEGs) of mRNAs were identified, including up-regulated secret phosphoprotein 1 (SPP1) and down-regulated pRB. Functional enrichment analysis revealed that while differential hypermethylation was related to cell cycle, intracellular part and protein binding, the main pathway involved herpes simplex virus 1 infection related to down-regulated AKT, Araf1 and BCL2A1. In the meantime, sexual reproduction, cohesin complex and protein C-terminus binding was functionally linked to differential hypomethylation, while fluid shear stress and atherosclerosis were identified as the main pathways related to up-regulated GST and CNP.

conclusionsWe showed that lung cancer development involved differential expression of SPP1 and pRB mRNA, as well as m6A mRNA methylation in AKT, APAF1, BCL2A1, GST and CNP genes.

Indexed as

DNA MethylationEpigenomeEpigenomicsGene Expression ProfilingOligonucleotide Array Sequence AnalysisTranscriptomeAdenosineBiomarkers, TumorGene Expression Regulation, NeoplasticGene Regulatory NetworksHumansLung NeoplasmsAdenosineBiomarkers, TumorN-methyladenosinegene ontologyKyoto Encyclopedia of Genes and Genomeslung cancerm6Amicroarray analysis

Identifiers

PMID34308964
PMCPMC8450313
OpenAlexW3185526033

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.