Evidence map›Paper›PMID 34308845›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2021

[Macrophage migration inhibitory factor meditates MPP+/MPTP-induced NLRP3 inflammasome activation in microglia cells].

H Huang, Y Gao, K Nie, L Wang

Open access · greenAbstract read
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In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
0.6field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

H HuangSchool of Medicine, South China University of Technology, Guangzhou 510006, China.
Y GaoDepartment of Neurology, Guangdong Provincial People's Hospital //Guangdong Neuroscience Institute, Guangzhou 510080, China.
K NieDepartment of Neurology, Guangdong Provincial People's Hospital //Guangdong Neuroscience Institute, Guangzhou 510080, China.
L WangDepartment of Neurology, Guangdong Provincial People's Hospital //Guangdong Neuroscience Institute, Guangzhou 510080, China.
South China University of Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo explore the mechanisms of macrophage migration inhibitory factor (MIF)/nucleus factor-κB (NF-κB) in mediating 1-methyl-4-phenylpyridinium iodide (MPP +)/1-Methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-induced activation of Nod-like receptor protein 3 (NLRP3) inflammasome in microglials and the its effects on neurons.

methodsMurine microglial cell line Bv-2 was infected with a lentivirus carrying MIF shRNA for MIF knockdown and then treated with MPP+. The total protein levels of NLRP3, caspase-1, p65 and p65 in the cell nuclei and cytoplasm were detected. ELISA was used to detect the levels of IL-1β and IL-18 in the culture supernatant, which served as the conditioned culture medium for MN9D cells, whose TH expression level was detected using Western blotting. The effect of stereotactic injection of an adeno-associated virus (AAV) carrying MIF shRNA on behaviors was assessed in a C57BL/6 mouse model of Parkinson disease (PD) induced by intraperitoneal MPTP injection. TH and Iba-1 immunohistochemistry was used to evaluate the number of substantia nigra neurons and the activation of microglia cells, and the protein expressions of MIF, NLRP3 and TH in the substantia nigra were detected with Western blotting.

resultsMPP+ significantly increased NLRP3 and MIF expressions in Bv-2 cells (

conclusionInhibition of MIF can reduce the expression of NLRP3 inflammasomes and inflammatory factor caused by MPP+ in microglia cells to relieve the damage of dopaminergic neurons and alleviate microglia cell activation, thus offering protection against neuroinflammation in Parkinson's disease.

Indexed as

InflammasomesMacrophage Migration-Inhibitory Factors1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine1-Methyl-4-phenylpyridiniumAnimalsDisease Models, AnimalDopaminergic NeuronsMaleMiceMice, Inbred C57BLMicrogliaMitochondrial Permeability Transition PoreNLR Family, Pyrin Domain-Containing 3 ProteinNLR Proteins1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine1-Methyl-4-phenylpyridiniumInflammasomesMacrophage Migration-Inhibitory FactorsMitochondrial Permeability Transition PoreNLR Family, Pyrin Domain-Containing 3 ProteinNlrp3 protein, mouseNLR Proteinsmacrophage migration inhibitory factormicroglia cellsneuroinflammationNod-like receptor protein 3 inflammasomeParkinson's disease

Identifiers

PMID34308845
PMCPMC8329680
OpenAlexW3183217986

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.