Evidence map›Paper›PMID 34308575›Full record

ArticleCell journal2021

Astaxanthin Protects Human Granulosa Cells against Oxidative Stress through Activation of NRF2/ARE Pathway and Its Downstream Phase II Enzymes.

Mojtaba Eslami, Sahar Esfandyari, Marzieh Aghahosseini, Zahra Rashidi, S Hirzad Hosseinishental, Samane Brenjian, Aligholi Sobhani, Fardin Amidi

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell journal, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 18 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Mojtaba EslamiDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Sahar EsfandyariDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Marzieh AghahosseiniDepartment of Infertility, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.
Zahra RashidiFertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
S Hirzad HosseinishentalDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Samane BrenjianDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Aligholi SobhaniDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Fardin AmidiDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. Email: famidi@sina.tums.ac.ir.
Tehran University of Medical Sciences · IRKermanshah University of Medical Sciences · IRShariati Hospital · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveAstaxanthin (AST) has been introduced as a radical scavenger and an anti-apoptotic factor that acts via regulating the nuclear factor-E2-related factor 2 (NRF2) and related factors. Here, we intended to examine the effect of AST on granulosa cells (GCs) against oxidative stress by examining NRF2 and downstream phase II antioxidant enzymes. MATERIALS AND

methodsIn this experimental study, we used cultured human primary GCs for the study. First, we performed the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test to evaluate cells viability after treatment with hydrogen peroxide (H

resultsThis study showed that AST suppressed ROS generation (P<0.01) and cell death (P<0.05) in GCs induced by oxidative stress. AST also elevated gene and protein expression and nuclear localization of NRF2 and had an inhibitory effect on the protein levels of KEAP1 (P<0.05). Furthermore, when we used trigonelline (Trig) as a known inhibitor of NRF2, it attenuated the protective effects of AST by decreasing NRF2 activity and gene expression of phase II enzymes (P<0.05).

conclusionOur results presented the protective role of AST against oxidative stress in GCs which was mediated through up-regulating the phase II enzymes as a result of NRF2 activation. Our study may help in improving

Indexed as

AstaxanthinGranulosa CellsNuclear Factor-E2-Related Factor 2Oxidative Stress

Identifiers

PMID34308575
PMCPMC8286460
OpenAlexW3183591297

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.