ReviewOpen veterinary journal
The MTT assay application to measure the viability of spermatozoa: A variety of the assay protocols.
Review in Open veterinary journal. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 87 citations in OpenAlex.
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- Investigating the Effects of Sea Anemone (Stichodactyla haddoni) Toxin on Potassium and Sodium Channel Gene Expression and Cell Death Mechanisms in A549 Cells.Marine biotechnology (New York, N.Y.) · 2026Article
- Preparation and bioactivity of probiotic-fermented lotus seed and lily bulb beverage.Frontiers in microbiology · 2026Article
- Effect of Enzymatic-based chemomechanical caries removal agent on proliferation and osteogenic differentiation of dental pulp stem cells.BMC oral health · 2025Article
- Polyherbal Therapeutics Mitigate CClReproductive sciences (Thousand Oaks, Calif.) · 2025Article
- Oxidative degradation of alpha-tocopherol by reactive oxygen species, identifying products, and product anticancer activity.BMC chemistry · 2025Article
- Eggplant (International journal of molecular sciences · 2025Article
- Optimizing Cell Density and Unveiling Cytotoxic Profiles of DMSO and Ethanol in Six Cancer Cell Lines: Experimental and In Silico Insights.Methods and protocols · 2025Article
- Atorvastatin-Loaded Carboxymethyl Cellulose-Gelatin Hydrogel: A Synergistic Strategy for Enhanced Wound Healing and Skin Tissue Regeneration.Iranian biomedical journal · 2025Article
- Inhibition of Inflammatory Regulators for Chronic Obstructive Pulmonary Disease (COPD) Treatment from Indonesian Medicinal Plants: A Systematic Review.Current issues in molecular biology · 2025Review
- Microneedle Delivery of Heterologous Microparticulate COVID-19 Vaccine Induces Cross Strain Specific Antibody Levels in Mice.Vaccines · 2025Article
- Pharmacological Inhibition of N-Acylethanolamine Acid Amidase (NAAA) Mitigates Intestinal Fibrosis Through Modulation of Macrophage Activity.Journal of Crohn's & colitis · 2025Article
- A fluorescent probe with a vanillin-pyridine-imidazole core structure for carboxylesterase detection in macrophage polarization during bone homeostasis.Frontiers in chemistry · 2025Article
- ABT-263, a BCL-2 inhibitor, selectively eliminates latently HIV-1-infected cells without viral reactivation.PloS one · 2025Article
- Toxicity and Dermatokinetic Analysis of Ibrutinib in Human Skin Models.Pharmaceutics · 2024Article
- Review
- Designing of a new transdermal antibiotic delivery polymeric membrane modified by functionalized SBA-15 mesoporous filler.Scientific reports · 2024Article
- Effect of tofacitinib on the phenotype and activity of Caco‑2 cells in a model of inflammatory bowel disease.Experimental and therapeutic medicine · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay is one of the methods used to evaluate the viability of sperm. In the assay, a tetrazolium component (MTT) is converted into MTT formazan by some specific enzymes in the viable cells. The amount of formazan product in theory is directly correlated with the percentage of viable sperms. It is quantified by measuring the absorbance using a spectrophotometer. The present article compiles the MTT assays that have been used to determine sperm viability in most animal species and humans. In each assay, other factors apart from the number of viable cells that potentially influence the accuracy and precision of results are stated, such as preparations of sperm and MTT solutions, length and conditions of incubation, and a solubilizing agent as well as the formazan detection method. Also, the strengths and shortcomings of the MTT test comparison with the others are summarized at the end of this article. This information may be useful for prospective researchers deciding to implement this colorimetric method in their experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.