Evidence map›Paper›PMID 34306109›Full record

ArticleIranian journal of veterinary research2021

Evaluation of the diagnostic potential and DIVA capability of recombinant LigBCon1-5 protein of

S K Behera, T C Sabarinath, Y Deneke, S K Bansal, K Mahendran, A Kumar, K Senthil, M R Verma, S ChandraSekar, S Atif Ali

Open access · greenAbstract read
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In one paragraph

Article in Iranian journal of veterinary research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.9field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Lateral flow assay for rapid serodiagnosis of bovine leptospirosis.Iranian journal of veterinary research · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 7 institutions in 3 countries.

S K BeheraDepartment of Epidemiology & Public Health, School of Life Science, Central University of Tamil Nadu, Tamil Nadu, India.
T C SabarinathClinical Bacteriological Laboratory, ICAR-Indian Veterinary Research Institute (ICAR-IVRI), Mukteshwar, India.
Y DenekeDepartment of Microbial Biotechnology, School of Veterinary Medicine, Jimma University, Jimma, Ethiopia.
S K BansalDepartment of Veterinary Medicine, Veterinary Clinical Complex, G C Negi College of Veterinary & Animal Sciences, Chaudhary Sarwan Kumar HP Krishi University, Palampur, Himachal Pradesh, India.
K MahendranVeterinary Medicine Division, ICAR-Indian Veterinary Research Institute (ICAR-IVRI), Izatnagar, Bareilly, India.
A KumarDivision of Veterinary Public Health, ICAR-Indian Veterinary Research Institute (ICAR-IVRI), Izatnagar, Bareilly, India.
K SenthilZoonosis Research Lab, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Tamil Nadu, India.
M R VermaLivestock Economics & Statistics Division, ICAR-Indian Veterinary Research Institute (ICAR-IVRI), Izatnagar, Bareilly, India.
S ChandraSekarBiochemistry Laboratory, ICAR-Indian Veterinary Research Institute (ICAR-IVRI), Mukteswar, India.
S Atif AliPh.D. Student in Biotechnology, Institute of Chemistry, Academia Sinica University, Nankang, Taipei, Taiwan.
Indian Veterinary Research Institute · INCentral University of Tamil Nadu · INChaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya · INIndian Council of Agricultural Research · INInstitute of Biological Chemistry, Academia Sinica · TWJimma University · ETTamil Nadu Veterinary and Animal Sciences University · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCanine leptospirosis is a serious public health concern.

aimsThis study aims to investigate the feasibility of conserved first to fifth domains of recombinant

methodsA total of 340 unvaccinated canine serum samples were screened using microscopic agglutination test (MAT) and rLigBCon1-5 based immunoglobulin G (IgG) indirect-enzyme-linked immunosorbent assay (I-ELISA). Further, 60 vaccinated canine sera were screened using MAT and rLigBCon1-5 based latex agglutination test (LAT).

resultsMicroscopic agglutination test results revealed seropositivity of 28.6%. The relative sensitivity, specificity, and accuracy of IgG I-ELISA in comparison to MAT were 100%, 96.0%, and 97.2%, respectively. Out of 60 vaccinated sera, 46 sera reacted with MAT alone, and eight sera reacted by both tests, while six sera were non-reactive with both tests. Anti-LigB antibodies were detected in eight canine sera by rLigBCon1-5 based LAT. In five LAT reactive sera, agglutinins of locally circulating

conclusionImmunoglobulin G based indirect ELISA assay (IgG I-ELISA) can be employed as an alternative test instead of MAT. rLigBCon1-5 based LAT detected anti-LigB antibodies in eight vaccinated sera where the vaccine failure occurred partially or totally due to the limited efficacy spectrum of Nobivac

Indexed as

Canine leptospirosisDIVA based testELISALatex agglutination testSpatial variation

Identifiers

PMID34306109
PMCPMC8294815
OpenAlexW3186498856

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.