ArticleFrontiers in microbiology2021
CRISPR-Based Isothermal Next-Generation Diagnostic Method for Virus Detection in Sugarbeet.
Article in Frontiers in microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 45 citations in OpenAlex.
- CRISPR-Cas systems for plant virus management: detection, surveillance, and host resistance.Frontiers in plant science · 2026Review
- Rapid and visual detection of Lentinula edodes spherical virus using CRISPR/Cas12a-based RT-RPA.Virus genes · 2025Article
- Single and dual RPA-CRISPR/Cas assays for point-of-need detection of Stewart's wilt pathogen (Pantoea stewartii subsp. stewartii) of corn and Maize dwarf mosaic virus.Pest management science · 2025Article
- CRISPR-Cas-Based Diagnosis of Geminiviruses.Methods in molecular biology (Clifton, N.J.) · 2025Article
- The rGO@AuNPs modified label-free electrochemical immunosensor to sensitive detection of CP-BNYVV protein of Rhizomania disease agent in sugar beet.Plant methods · 2024Article
- A novel system with robust compatibility and stability for detectingMicrobiology spectrum · 2024Article
- Advances in plant pathogen detection: integrating recombinase polymerase amplification with CRISPR/Cas systems.3 Biotech · 2024Review
- CRISPR/Cas12a-based method coupled with isothermal amplification to identifyFrontiers in microbiology · 2024Article
- Recent advances and challenges in plant viral diagnostics.Frontiers in plant science · 2024Review
- Advancements and prospects of CRISPR/Cas9 technologies for abiotic and biotic stresses in sugar beet.Frontiers in genetics · 2023Review
- Advancements and prospectives of sugar beet (Beta vulgaris L.) biotechnology.Applied microbiology and biotechnology · 2022Review
- Onsite detection of plant viruses using isothermal amplification assays.Plant biotechnology journal · 2022Review
- Diagnostics of Infections Produced by the Plant Viruses TMV, TEV, and PVX with CRISPR-Cas12 and CRISPR-Cas13.ACS synthetic biology · 2022Article
- Rapid detection of genetically modified products based on CRISPR-Cas12a combined with recombinase polymerase amplification.Current research in food science · 2022Article
- Sensitive and Rapid Detection of Citrus Scab Using an RPA-CRISPR/Cas12a System Combined with a Lateral Flow Assay.Plants (Basel, Switzerland) · 2021Article
- CRISPR-Cas-Led Revolution in Diagnosis and Management of Emerging Plant Viruses: New Avenues Toward Food and Nutritional Security.Frontiers in nutrition · 2021Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rhizomania is a disease of sugarbeet caused by beet necrotic yellow vein virus (BNYVV) that significantly affects sugarbeet yield globally. Accurate and sensitive detection methods for BNYVV in plants and field soil are necessary for growers to make informed decisions on variety selection to manage this disease. A recently developed CRISPR-Cas-based detection method has proven highly sensitive and accurate in human virus diagnostics. Here, we report the development of a CRISPR-Cas12a-based method for detecting BNYVV in the roots of sugarbeet. A critical aspect of this technique is the identification of conditions for isothermal amplification of viral fragments. Toward this end, we have developed a reverse transcription (RT) recombinase polymerase amplification (RPA) for detecting BNYVV in sugarbeet roots. The RT-RPA product was visualized, and its sequence was confirmed. Subsequently, we designed and validated the cutting efficiency of guide RNA targeting BNYVV via
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