Evidence map›Paper›PMID 34305650›Full record

ArticleFrontiers in physiology2021

Whole-Mount Kidney Clearing and Visualization Reveal Renal Sympathetic Hyperinnervation in Heart Failure Mice.

Chao Wu, Fang Yan, Min Li, Yimin Tu, Ziyu Guo, Yufei Chen, Yaxin Wu, Qing Li, Changan Yu, Yi Fu and 5 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in physiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed, 1 pooled it
0.6field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 1 synthesis or guideline pooled it, 6 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 5 institutions in 1 country.

Chao WuDepartment of Cardiology, Peking University China-Japan Friendship School of Clinical Medicine, Beijing, China.
Fang YanSchool of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Min LiSchool of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Yimin TuGraduate School, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Ziyu GuoDepartment of Cardiology, Peking University China-Japan Friendship School of Clinical Medicine, Beijing, China.
Yufei ChenDepartment of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing, China.
Yaxin WuDepartment of Cardiology, Peking University China-Japan Friendship School of Clinical Medicine, Beijing, China.
Qing LiDepartment of Cardiology, Peking University China-Japan Friendship School of Clinical Medicine, Beijing, China.
Changan YuDepartment of Cardiology, China-Japan Friendship Hospital, Beijing, China.
Yi FuDepartment of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing, China.
Meihui WuDepartment of Nursing, Army Medical Center of PLA, Chongqing, China.
Wei KongDepartment of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing, China.
Yanxiang GaoDepartment of Cardiology, China-Japan Friendship Hospital, Beijing, China.
Xiaowei LiSchool of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Jingang ZhengDepartment of Cardiology, Peking University China-Japan Friendship School of Clinical Medicine, Beijing, China.
China-Japan Friendship Hospital · CNPeking University · CNShanghai Jiao Tong University · CNArmy Medical University · CNMinistry of Education of the People's Republic of China · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Developing a three-dimensional (3D) visualization of the kidney at the whole-mount scale is challenging. In the present study, we optimized mouse whole-mount kidney clearing, which improved the transparency ratio to over 90% based on organ-specific perfusion (OSP)-clear, unobstructed brain imaging cocktails and computational analysis (CUBIC). The optimized OSP-CUBIC-compatible 3D immunostaining and imaging simultaneously visualized the high-resolution 3D structure of the whole-mount renal microvascular, glomerulus, and accompanying wrapped traveling sympathetic nerves in mice. A mouse model of pressure overload-induced heart failure (HF) was then established by minimally invasive transverse aortic constriction (MTAC). Further 3D quantification revealed renal sympathetic hyperinnervation (6.80 ± 1.04%

Indexed as

3D imagingCUBICheart failurerenal dysfunctionsympathetic nervetissue clearing

Identifiers

PMID34305650
PMCPMC8297621
OpenAlexW3180656877

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.