ArticleBiomaterials2021
A robust vasculogenic microfluidic model using human immortalized endothelial cells and Thy1 positive fibroblasts.
Article in Biomaterials, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 54 citations in OpenAlex.
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- Photo-Responsive Functional Hydrogels: Mechanism, Design, and Application.Chemistry, an Asian journal · 2026Review
- Insulin-like growth factor-binding protein 7 is a soluble cell adhesion molecule.Science advances · 2026Article
- Immortalized smooth muscle cells enhance in vitro vasculogenesis.Angiogenesis · 2026Article
- Computational fluid dynamics enables predictable scale-up of perfusion bioreactors for microvessel production.PNAS nexus · 2026Article
- IntravChip: a vascularized and perfused microfluidic model of the primary tumor microenvironment to collect intravasated tumor cells.Biofabrication · 2026Article
- Development of a Synthetic Hydrogel to Foster Microvascularization of an Endometriosis Microphysiological System.Advanced healthcare materials · 2026Article
- Immortalized smooth muscle cells enhance in vitro vasculogenesis.Research square · 2026Article
- ETV2 Mediated Differentiation of Human Pluripotent Stem Cells Results in Functional Endothelial Cells for Engineering Advanced Vascularized Microphysiological Models.Advanced healthcare materials · 2026Article
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- IntravChip: a vascularized and perfused microfluidic model of the primary tumor microenvironment to collect intravasated tumor cells.bioRxiv : the preprint server for biology · 2026Article
- Ether lipids influence cancer cell fate by modulating iron uptake.Nature communications · 2026Article
- Three-dimensional modeling of flow through microvascular beds and surrounding interstitial spaces.Bioengineering & translational medicine · 2026Article
- Time and architecture: the next two dimensions of microphysiological systems.npj biomedical innovations · 2026Review
- Ether lipids influence cancer cell fate by modulating iron uptake.bioRxiv : the preprint server for biology · 2025Article
- Multidirectional interstitial flow promotes microvascular network formation: insights from a square chip-based platform.Angiogenesis · 2025Article
- Fabricating Microfluidic Co-Cultures of Immortalized Cell Lines Uncovers Robust Design Principles for the Simultaneous Formation of Patterned, Vascularized, and Stem Cell-Derived Adipose Tissue.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Polyploidy of MDA-MB-231 cells drives increased extravasation with enhanced cell-matrix adhesion.APL bioengineering · 2025Article
- Long-term physiological flow rescues regressed microvascular networks and increases their longevity.npj biological physics and mechanics · 2025Article
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Authors and funding
14 authors at 3 institutions in 2 countries.
Funding
Abstract
Human umbilical vein endothelial cells (HUVECs) and stromal cells, such as human lung fibroblasts (FBs), have been widely used to generate functional microvascular networks (μVNs) in vitro. However, primary cells derived from different donors have batch-to-batch variations and limited lifespans when cultured in vitro, which hampers the reproducibility of μVN formation. Here, we immortalize HUVECs and FBs by exogenously expressing human telomerase reverse transcriptase (hTERT) to obtain stable endothelial cell and FB sources for μVN formation in vitro. Interestingly, we find that immortalized HUVECs can only form functional μVNs with immortalized FBs from earlier passages but not from later passages. Mechanistically, we show that Thy1 expression decreases in FBs from later passages. Compared to Thy1 negative FBs, Thy1 positive FBs express higher IGFBP2, IGFBP7, and SPARC, which are important for angiogenesis and lumen formation during vasculogenesis in 3D. Moreover, Thy1 negative FBs physically block microvessel openings, reducing the perfusability of μVNs. Finally, by culturing immortalized FBs on gelatin-coated surfaces in serum-free medium, we are able to maintain the majority of Thy1 positive immortalized FBs to support perfusable μVN formation. Overall, we establish stable cell sources for μVN formation and characterize the functions of Thy1 positive and negative FBs in vasculogenesis in vitro.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.