Evidence map›Paper›PMID 34303064›Full record

ArticleBioelectrochemistry (Amsterdam, Netherlands)2021

Real-time impedance feedback to enhance cutaneous gene electrotransfer in a murine skin model.

Reginald M Atkins, Timothy J Fawcett, Richard Gilbert, Andrew M Hoff, Richard Connolly, Douglas W Brown, Mark J Jaroszeski

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioelectrochemistry (Amsterdam, Netherlands), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Reginald M AtkinsDepartment of Chemical, Biological, and Materials Engineering, University of South Florida, 4202 E. Fowler Ave ENG 030, Tampa, FL 33620, USA; Center for Molecular Delivery at USF, University of South Florida, 4202 E. Fowler Ave ENG 030, Tampa, FL 33620, USA.
Timothy J FawcettDepartment of Chemical, Biological, and Materials Engineering, University of South Florida, 4202 E. Fowler Ave ENG 030, Tampa, FL 33620, USA; Center for Molecular Delivery at USF, University of South Florida, 4202 E. Fowler Ave ENG 030, Tampa, FL 33620, USA; Research Computing, University of South Florida, 4202 E. Fowler Ave ENG 030, Tampa, FL 33620, USA.
Richard GilbertDepartment of Chemical, Biological, and Materials Engineering, University of South Florida, 4202 E. Fowler Ave ENG 030, Tampa, FL 33620, USA; Center for Molecular Delivery at USF, University of South Florida, 4202 E. Fowler Ave ENG 030, Tampa, FL 33620, USA.
Andrew M HoffCenter for Molecular Delivery at USF, University of South Florida, 4202 E. Fowler Ave ENG 030, Tampa, FL 33620, USA; Department of Electrical Engineering, University of South Florida, 4202 E. Fowler Ave ENG 030, Tampa, FL 33620, USA.
Richard ConnollyDepartment of Chemical, Biological, and Materials Engineering, University of South Florida, 4202 E. Fowler Ave ENG 030, Tampa, FL 33620, USA; Center for Molecular Delivery at USF, University of South Florida, 4202 E. Fowler Ave ENG 030, Tampa, FL 33620, USA.
Douglas W BrownSchool of Electrical and Computer Engineering, Georgia Institute of Technology, 777 Atlantic Dr. NW, Atlanta, GA 30332, USA.
Mark J JaroszeskiDepartment of Chemical, Biological, and Materials Engineering, University of South Florida, 4202 E. Fowler Ave ENG 030, Tampa, FL 33620, USA; Center for Molecular Delivery at USF, University of South Florida, 4202 E. Fowler Ave ENG 030, Tampa, FL 33620, USA; Department of Medical Engineering, University of South Florida, 4202 E. Fowler Avenue ENG 030, Tampa, FL 33620, USA. Electronic address: mjarosze@usf.edu.

Funding

Regulated Heat Combined with Impedance Spectroscopy to Improve and Control Electroporative DNA Delivery to the Skin in VivoR01EB027497 · NIBIB · UNIVERSITY OF SOUTH FLORIDA · PI JAROSZESKI, MARK J. · 2019 to 2022
$1.6M
Impedance Changes as an Indicator of Successful Skin Electroporative DNA deliveryR21AR061136 · NIAMS · UNIVERSITY OF SOUTH FLORIDA · PI JAROSZESKI, MARK J. · 2011 to 2012
$354k
NIAMS NIH HHS R21 AR061136NIBIB NIH HHS R01 EB027497
6 · The paper itself

Abstract

Electric field mediated gene delivery methods have the ability to efficiently transfect cells in vivo with an excellent safety profile. The method has historically used a fixed number of electric pulses with identical characteristics in induce delivery. Electrical treatment does not typically compensate for subject-to-subject variation and other differences. This study was designed to investigate if delivery/expression could be increased using a novel electropulsation method that compensated for variation using real-time electrical impedance measurements. The method involved delivering plasmid DNA encoding luciferase to murine skin. Tissue impedance in a 1-3 KHz range was measured before electric pulses were applied. Impedance was also measured after each successive pulse. Pulsation was stopped when impedance values were reduced by either 80% or 95% relative to prepulse values. Standard/fixed pulsing parameters were also used for comparison. The results indicated that up to 15-fold increases in luciferase expression could be obtained when electrical treatment was ceased based upon impedance reductions. Furthermore, peak expression levels of all treatment groups pulsed using the novel pulsing method were statistically higher than those that employed standard pulsing. These results strongly suggest that applying pulses until a defined impedance-based endpoint results in higher expression.

Indexed as

Electric ImpedanceAnimalsElectroporationFemaleGenetic TherapyLuciferasesMaleMiceMice, Inbred BALB CSkinLuciferasesElectroporationGene deliveryGene electrotransferGene therapySkin

Identifiers

PMID34303064
PMCPMC8502206

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.