Evidence map›Paper›PMID 34302663›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Generation of Efficient Knock-in Mouse and Human Pluripotent Stem Cells Using CRISPR-Cas9.

Tatsuya Anzai, Hiromasa Hara, Nawin Chanthra, Taketaro Sadahiro, Masaki Ieda, Yutaka Hanazono, Hideki Uosaki

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Tatsuya Anzai *Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, Tochigi, Japan.
Hiromasa Hara *Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, Tochigi, Japan.
Nawin ChanthraDivision of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, Tochigi, Japan.
Taketaro SadahiroDepartment of Cardiology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.
Masaki IedaDepartment of Cardiology, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.
Yutaka HanazonoDivision of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, Tochigi, Japan.
Hideki UosakiDivision of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, Tochigi, Japan. uosaki.hideki@jichi.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A knock-in can generate fluorescent or Cre-reporter under the control of an endogenous promoter. It also generates knock-out or tagged-protein with fluorescent protein and short tags for tracking and purification. Recent advances in genome editing with clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated protein 9 (Cas9) significantly increased the efficiencies of making knock-in cells. Here we describe the detailed protocols of generating knock-in mouse and human pluripotent stem cells (PSCs) by electroporation and lipofection, respectively.

Indexed as

CRISPR-Cas SystemsAnimalsCells, CulturedClone CellsCulture MediaDNA PrimersDrug ResistanceElectroporationEmbryonic Stem CellsGene EditingGene Knock-In TechniquesGenes, ReporterHumansInduced Pluripotent Stem CellsMiceMice, Inbred C57BLCulture MediaDNA PrimersPuromycinRNA, Guide, CRISPR-Cas SystemsCRISPR-Cas9Genome editingHomology-directed repairKnock-inPluripotent stem cells

Identifiers

PMID34302663

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.