Evidence map›Paper›PMID 34302646›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Isolation of Cardiomyocytes Derived from Human Pluripotent Stem Cells Using miRNA Switches.

Kenji Miki, Hirohide Saito, Yoshinori Yoshida

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Kenji MikiDepartment of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan.
Hirohide SaitoDepartment of Life Science Frontiers, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan. hirohide.saito@cira.kyoto-u.ac.jp.
Yoshinori YoshidaCenter for iPS Cell Research and Application, Kyoto University, Kyoto, Japan. yoshinor@cira.kyoto-u.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The most common method for isolating cells of interest is an antibody method that recognizes cell surface antigens. However, specific surface antigens for many cell types have not been identified. We have developed the microRNA (miRNA)-responsive synthetic mRNA systems (miRNA switches), which isolate target cells based on endogenous miRNA activity. In this chapter, we describe protocols for isolating human pluripotent stem cell (hPSC)-derived cardiomyocytes using miRNA switches with or without cell sorting.

Indexed as

Cell Culture TechniquesCell DifferentiationCell LineCell SeparationFlow CytometryHumansMicroRNAsMyocytes, CardiacPluripotent Stem CellsRNA, MessengerMicroRNAsRNA, MessengerCardiomyocytesHuman pluripotent stem cellsIsolationmiRNA switchSynthetic modified mRNA

Identifiers

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.