Evidence map›Paper›PMID 34302534›Full record

ArticleApplied microscopy2021

Multispectral intravital microscopy for simultaneous bright-field and fluorescence imaging of the microvasculature.

Barry G H Janssen, Mohamadreza Najiminaini, Yan Min Zhang, Parsa Omidi, Jeffrey J L Carson

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microscopy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.2field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 2 countries.

Barry G H JanssenDepartment of Medical Biophysics, Western University, London, ON, N6A 5C1, Canada. gjansse@uwo.ca.ORCID http://orcid.org/0000-0003-3063-8200
Mohamadreza NajiminainiImaging Program, St.Joseph's Health Care, Lawson Health Research Institute, London, ON, N6A 4V2, Canada.
Yan Min ZhangKidney Clinical Research Unit (KCRU), London Health Sciences Centre, London, ON, N6C 6B5, Canada.
Parsa OmidiImaging Program, St.Joseph's Health Care, Lawson Health Research Institute, London, ON, N6A 4V2, Canada.
Jeffrey J L CarsonDepartment of Medical Biophysics, Western University, London, ON, N6A 5C1, Canada.
Western University · CA

Funding

Lawson Health Research Institute (CA) N.A.Schulich School of Medicine and Dentistry N.A.
6 · The paper itself

Abstract

Intravital video microscopy permits the observation of microcirculatory blood flow. This often requires fluorescent probes to visualize structures and dynamic processes that cannot be observed with conventional bright-field microscopy. Conventional light microscopes do not allow for simultaneous bright-field and fluorescent imaging. Moreover, in conventional microscopes, only one type of fluorescent label can be observed. This study introduces multispectral intravital video microscopy, which combines bright-field and fluorescence microscopy in a standard light microscope. The technique enables simultaneous real-time observation of fluorescently-labeled structures in relation to their direct physical surroundings. The advancement provides context for the orientation, movement, and function of labeled structures in the microcirculation.

Indexed as

Brightfield microscopyFluorescence microscopyIntravital microscopyMultispectral microscopy

Identifiers

PMID34302534
PMCPMC8310548
OpenAlexW3184844207

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.