Evidence map›Paper›PMID 34302317›Full record

ArticleAngewandte Chemie (International ed. in English)2021

Programmable Site-Specific Functionalization of DNA Origami with Polynucleotide Brushes.

Yunqi Yang, Qinyi Lu, Chao-Min Huang, Hongji Qian, Yunlong Zhang, Sonal Deshpande, Gaurav Arya, Yonggang Ke, Stefan Zauscher

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Article
  2. Tunable Patterning of DNA Origami on Surfaces Using Steric Brushes.Angewandte Chemie (International ed. in English) · 2026
    Article
  3. Review
  4. Review
  5. Article
  6. Article
  7. Article
  8. Controlling Silicification on DNA Origami with Polynucleotide Brushes.Journal of the American Chemical Society · 2024
    Article
  9. Piggybacking functionalized DNA nanostructures into live cell nuclei.bioRxiv : the preprint server for biology · 2024
    Article
  10. Review
  11. Article
  12. Review
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yunqi YangDepartment of Mechanical Engineering and Materials Science, Duke University, Durham, NC, 27708, USA.ORCID 0000-0002-6668-2628
Qinyi LuDepartment of Chemistry, Emory University, Atlanta, GA, 30322, USA.
Chao-Min HuangDepartment of Mechanical Engineering and Materials Science, Duke University, Durham, NC, 27708, USA.
Hongji QianDepartment of Biomedical Engineering, Duke University, Durham, NC, 27708, USA.
Yunlong ZhangDepartment of Chemistry, Emory University, Atlanta, GA, 30322, USA.
Sonal DeshpandeDepartment of Biomedical Engineering, Duke University, Durham, NC, 27708, USA.
Gaurav AryaDepartment of Mechanical Engineering and Materials Science, Duke University, Durham, NC, 27708, USA.
Yonggang KeDepartment of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA, 30322, USA.
Stefan ZauscherDepartment of Mechanical Engineering and Materials Science, Duke University, Durham, NC, 27708, USA.ORCID 0000-0002-2290-7178

Funding

In situ Enzymatic Synthesis of Aptamer Targeted Polynucleotide Drug Nanoparticles for Cancer TherapyR21EB026590 · NIBIB · DUKE UNIVERSITY · PI ZAUSCHER, STEFAN · 2018 to 2020
$621k
national science foundation ACI-1053575national science foundation DMR-1654485national science foundation ECCS-1807568NIBIB NIH HHS R21 EB026590NIH HHS EB026590-03u.s. department of energy DE-SC0020996
6 · The paper itself

Abstract

Combining surface-initiated, TdT (terminal deoxynucleotidyl transferase) catalyzed enzymatic polymerization (SI-TcEP) with precisely engineered DNA origami nanostructures (DONs) presents an innovative pathway for the generation of stable, polynucleotide brush-functionalized DNA nanostructures. We demonstrate that SI-TcEP can site-specifically pattern DONs with brushes containing both natural and non-natural nucleotides. The brush functionalization can be precisely controlled in terms of the location of initiation sites on the origami core and the brush height and composition. Coarse-grained simulations predict the conformation of the brush-functionalized DONs that agree well with the experimentally observed morphologies. We find that polynucleotide brush-functionalization increases the nuclease resistance of DONs significantly, and that this stability can be spatially programmed through the site-specific growth of polynucleotide brushes. The ability to site-specifically decorate DONs with brushes of natural and non-natural nucleotides provides access to a large range of functionalized DON architectures that would allow for further supramolecular assembly, and for potential applications in smart nanoscale delivery systems.

Indexed as

Deoxyuracil NucleotidesDNADNA NucleotidylexotransferaseNanostructuresNucleic Acid ConformationPolymerizationPolynucleotidesProof of Concept StudyThymine Nucleotides2'-deoxy-2'-fluorouridine-5'-triphosphateDeoxyuracil NucleotidesDNADNA NucleotidylexotransferasePolynucleotidesthymidine 5'-triphosphateThymine NucleotidesDNA nanotechnologydrug deliverymolecular dynamics simulationsnuclease resistancesurface-initiated polymerization

Identifiers

PMID34302317
PMCPMC8511278

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.