ArticleCommunications biology2021
3D test sample for the calibration and quality control of stimulated emission depletion (STED) and confocal microscopes.
Article in Communications biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed.
- Quantitative 3D Real-Space Analysis of Photonic Supraparticles.Advanced materials (Deerfield Beach, Fla.) · 2026Article
- STED Super-Resolution Microscopy for Studying Actin Cytoskeleton Organization.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Innovative sample preparation using alcohol dehydration and high refractive index medium enables acquisition of two-channel super-resolution 3D STED image of an entire oocyte.Journal of microscopy · 2025Article
- Enabling three-dimensional real-space analysis of ionic colloidal crystallization.Nature materials · 2024Article
- Electron-beam patterned calibration structures for structured illumination microscopy.Scientific reports · 2022Article
- Recent advances in the standardization of fluorescence microscopy for quantitative image analysis.Biophysical reviews · 2022Review
Corrections and comments
- Erratum issued
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multiple samples are required to monitor and optimize the quality and reliability of quantitative measurements of stimulated emission depletion (STED) and confocal microscopes. Here, we present a single sample to calibrate these microscopes, align their laser beams and measure their point spread function (PSF) in 3D. The sample is composed of a refractive index matched colloidal crystal of silica beads with fluorescent and gold cores. The microscopes can be calibrated in three dimensions using the periodicity of the crystal; the alignment of the laser beams can be checked using the reflection of the gold cores; and the PSF can be measured at multiple positions and depths using the fluorescent cores. It is demonstrated how this sample can be used to visualize and improve the quality of STED and confocal microscopy images. The sample is adjustable to meet the requirements of different NA objectives and microscopy techniques and additionally can be used to evaluate refractive index mismatches as a function of depth quantitatively.
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Registered trials
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