ArticleScientific reports2021
Manipulation of the Tyrosinase gene permits improved CRISPR/Cas editing and neural imaging in cichlid fish.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Complex pectoral-fin-driven locomotion underlies refined visuomotor behavior in the cichlidiScience · 2026Article
- Cell-type-specific architecture of the hypothalamus in a socially plastic vertebrate.bioRxiv : the preprint server for biology · 2026Article
- Advances in Fish Gene Editing.Animals : an open access journal from MDPI · 2026Review
- Cichlid fishes.Nature methods · 2025Article
- A pheromone receptor in cichlid fish mediates attraction to females but inhibits male parental care.Current biology : CB · 2024Article
- The dwarf neon rainbowfish Melanotaenia praecox, a small spiny-rayed fish with potential as a new Acanthomorpha model fish: I. Fin ray ontogeny and postembryonic staging.Developmental dynamics : an official publication of the American Association of Anatomists · 2024Article
- The dwarf neon rainbowfish Melanotaenia praecox, a small spiny-rayed fish with potential as a new Acanthomorpha model fish: II. Establishment of a microinjection procedure for genetic engineering.Developmental dynamics : an official publication of the American Association of Anatomists · 2024Article
- Review
- Genome editing in East African cichlids and tilapias: state-of-the-art and future directions.Open biology · 2023Review
- Pheromone Perception in Fish: Mechanisms and Modulation by Internal Status.Integrative and comparative biology · 2023Review
- Morphological and temporal variation in early embryogenesis contributes to species divergence in Malawi cichlid fishes.Evolution & development · 2023Article
- East African cichlid fishes.EvoDevo · 2023Review
- Article
- An intronic transposon insertion associates with a trans-species color polymorphism in Midas cichlid fishes.Nature communications · 2022Article
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Authors and funding
5 authors.
Funding
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Abstract
Direct tests of gene function have historically been performed in a limited number of model organisms. The CRISPR/Cas system is species-agnostic, offering the ability to manipulate genes in a range of models, enabling insights into evolution, development, and physiology. Astatotilapia burtoni, a cichlid fish from the rivers and shoreline around Lake Tanganyika, has been extensively studied in the laboratory to understand evolution and the neural control of behavior. Here we develop protocols for the creation of CRISPR-edited cichlids and create a broadly useful mutant line. By manipulating the Tyrosinase gene, which is necessary for eumelanin pigment production, we describe a fast and reliable approach to quantify and optimize gene editing efficiency. Tyrosinase mutants also remove a major obstruction to imaging, enabling visualization of subdermal structures and fluorophores in situ. These protocols will facilitate broad application of CRISPR/Cas9 to studies of cichlids as well as other non-traditional model aquatic species.
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